ELAVL2‐directed RNA regulatory network drives the formation of quiescent primordial follicles
Formation of primordial follicles is a fundamental, early process in mammalian oogenesis. However, little is known about the underlying mechanisms. We herein report that the RNA‐binding proteins ELAVL2 and DDX6 are indispensable for the formation of quiescent primordial follicles in mouse ovaries. W...
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Published in | EMBO reports Vol. 20; no. 12; pp. e48251 - n/a |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Blackwell Publishing Ltd
05.12.2019
John Wiley and Sons Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Formation of primordial follicles is a fundamental, early process in mammalian oogenesis. However, little is known about the underlying mechanisms. We herein report that the RNA‐binding proteins ELAVL2 and DDX6 are indispensable for the formation of quiescent primordial follicles in mouse ovaries. We show that Elavl2 knockout females are infertile due to defective primordial follicle formation. ELAVL2 associates with mRNAs encoding components of P‐bodies (cytoplasmic RNP granules involved in the decay and storage of RNA) and directs the assembly of P‐body‐like granules by promoting the translation of DDX6 in oocytes prior to the formation of primordial follicles. Deletion of Ddx6 disturbs the assembly of P‐body‐like granules and severely impairs the formation of primordial follicles, indicating the potential importance of P‐body‐like granules in the formation of primordial follicles. Furthermore, Ddx6‐deficient oocytes are abnormally enlarged due to misregulated PI3K‐AKT signaling. Our data reveal that an ELAVL2‐directed post‐transcriptional network is essential for the formation of quiescent primordial follicles.
Synopsis
The RNA‐binding proteins ELAVL2 and DDX6 drive the assembly of large P body‐like cytoplasmic RNP granules in oocytes and the formation of quiescent primordial follicles.
ELAVL2 is required for the formation of primordial follicles in mice.
Large P body‐like granules are assembled in oocytes prior to the formation of primordial follicles in an ELAVL2‐dependent manner.
ELAVL2‐dependent DDX6 translation is crucial for the assembly of P body‐like granules.
DDX6 is required for maintaining the quiescent state of primordial follicles.
The RNA‐binding proteins ELAVL2 and DDX6 drive the assembly of large P body‐like cytoplasmic RNP granules in oocytes and the formation of quiescent primordial follicles. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1469-221X 1469-3178 |
DOI: | 10.15252/embr.201948251 |