Identification of a promoter for the vegetative insecticidal protein-encoding gene vip3LB from Bacillus thuringiensis

• Published in: FEMS microbiology letters Vol. 247; no. 1; pp. 101 - 104
• Main Authors: Mesrati, Lobna Abdelkefi, Tounsi, Slim, Kamoun, Fakher, Jaoua, Samir
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.06.2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Bacillus thuringiensis; Bacillus thuringiensis - genetics; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Base Sequence; Biological and medical sciences; Blotting, Northern; Conserved sequence; Expression; Fundamental and applied biological sciences. Psychology; Gene expression; Genetics; Homology; Microbiology; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nucleotide sequence; Nucleotides; Promoter; Promoter Regions, Genetic; Proteins; Sequence Alignment; Sporulation; Transcription; Transcription Initiation Site; Transcription start point; Transcription. Transcription factor. Splicing. Rna processing; vip3 gene; Promoter; Transcription start point; Expression; vip3 gene; Bacillus thuringiensis; gene; Primer extension technique; Insecticide; Transcription; Pesticides; Sequence alignment; Identification; Bacillaceae; Characterization; Northern blotting; Bacillales; Bacteria; Transcription factor
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/j.femsle.2005.04.032

The expression of vip3LB, one of the vegetative insecticidal protein-encoding genes of Bacillus thuringiensis, was studied at the transcriptional level. By primer extension analysis, we have identified, for the first time, the transcription start point of vip3-type gene. Upstream from vip3LB transcription start point, was found a nucleotide sequence partially homologous to the consensus sequence for the E σ E holoenzyme of B. subtilis. Thus, it was strongly suggested that the identified vip3 promoter was under the control of σ 35-like enzyme, the B. thuringiensis homolog of σ E. The transcriptional activity from the promoter was detected at the vegetative stage of growth starting at mid-log phase as well as during the middle stage of sporulation.