Molecular cloning and tissue distribution of cholecystokinin-1 receptor (CCK-1R) in yellowtail Seriola quinqueradiata and its response to feeding and in vitro CCK treatment

• Published in: General and comparative endocrinology Vol. 186; pp. 1 - 8
• Main Authors: Furutani, Takahiro, Masumoto, Toshiro, Fukada, Haruhisa
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2013
• Subjects: Animals; cecum; Cholecystokinin; Cholecystokinin - pharmacology; cholecystokinin receptors; Cholecystokinin-1 receptor; Cholecystokinin-A receptor; Cloning, Molecular; complementary DNA; digestion; digestive enzymes; digestive tract; Endocrinology & Metabolism; fish; gall bladder; Gallbladder - drug effects; Gallbladder - metabolism; gene expression; in vivo studies; Intestines - drug effects; Intestines - metabolism; mammals; Marine; messenger RNA; molecular cloning; Perciformes - genetics; Perciformes - metabolism; Phylogeny; Receptors, Cholecystokinin - chemistry; Receptors, Cholecystokinin - classification; Receptors, Cholecystokinin - genetics; Receptors, Cholecystokinin - metabolism; Reverse Transcriptase Polymerase Chain Reaction; sequence analysis; Seriola quinqueradiata; tissue distribution; Yellowtail; Cholecystokinin-A receptor; Yellowtail; Cholecystokinin-1 receptor; Cholecystokinin
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2013.02.003

Highlights ► Yellowtail CCK-1R cDNA was cloned. ► High expression levels of CCK-1R mRNA was observed in digestive tracts. ► CCK-1R, CCK and digestive enzymes mRNA levels increased after feeding. ► CCK-1R and digestive enzymes increased by addition of CCK in vitro experiment. ► CCK-1R plays an important role in digestion stimulated by CCK in yellowtail.