Plant Platforms for Efficient Heterologous Protein Production

• Published in: Biotechnology and bioprocess engineering Vol. 26; no. 4; pp. 546 - 567
• Main Authors: Ghag, Siddhesh B., Adki, Vinayak S., Ganapathi, Thumballi R., Bapat, Vishwas A.
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.08.2021; Springer Nature B.V; 한국생물공학회
• Subjects: agroinfiltration; Antibodies; bioprocessing; Biotechnology; Chemistry; Chemistry and Materials Science; Clinical trials; Genetic transformation; heterologous gene expression; Industrial and Production Engineering; Insects; production costs; protein synthesis; Proteins; Quality standards; Review Paper; therapeutics; Vaccines; 생물공학; plantibodies; therapeutics; magnifection; vaccines; protein; biopharmaceuticals; clinical trials
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-020-0374-1

Production of recombinant proteins is primarily established in cultures of mammalian, insect and bacterial cells. Concurrently, concept of using plants to produce high-value pharmaceuticals such as vaccines, antibodies, and dietary proteins have received worldwide attention. Newer technologies for plant transformation such as plastid engineering, agroinfiltration, magnifection, and deconstructed viral vectors have been used to enhance the protein production in plants along with the inherent advantage of speed, scale, and cost of production in plant systems. Production of therapeutic proteins in plants has now a more pragmatic approach when several plant-produced vaccines and antibodies successfully completed Phase I clinical trials in humans and were further scheduled for regulatory approvals to manufacture clinical grade products on a large scale which are safe, efficacious, and meet the quality standards. The main thrust of this review is to summarize the data accumulated over the last two decades and recent development and achievements of the plant derived therapeutics. It also attempts to discuss different strategies employed to increase the production so as to make plants more competitive with the established production systems in this industry.