Contribution of ultrasound and slightly acid electrolytic water combination on inactivating Rhizopus stolonifer in sweet potato
•Ultrasound (US) and slightly acidic electrolyzed water (SAEW) were combined.•US + SAEW treatment inhibited mycelial growth and spore germination of R. stolonifer.•US + SAEW treatment disrupted the cell membranes and caused the leakage of cytoplasm.•US500 + SAEW50 at 40 and 55 °C increased cell memb...
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Published in | Ultrasonics sonochemistry Vol. 73; p. 105528 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.05.2021
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | •Ultrasound (US) and slightly acidic electrolyzed water (SAEW) were combined.•US + SAEW treatment inhibited mycelial growth and spore germination of R. stolonifer.•US + SAEW treatment disrupted the cell membranes and caused the leakage of cytoplasm.•US500 + SAEW50 at 40 and 55 °C increased cell membrane permeability and decreased MMP.•US500 + SAEW50 at 40 °C and US300 + SAEW50 at 55 °C controlled soft rot decay in TRs.
Effects of ultrasound (US, 300, 400, and 500 W) and slightly acidic electrolyzed water (SAEW, 10, 30, and 50 mg/L) combination on inactivating Rhizopus stolonifer in sweet potato tuberous roots (TRs) were investigated. US at 300, 400, and 500 W simultaneous SAEW with available chlorine concentration of 50 mg/L at 40 and 55 °C for 10 min significantly inhibited colony diameters (from 90.00 to 6.00–71.62 mm) and spores germination (p < 0.05). US + SAEW treatment could destroy cell membrane integrity and lead to the leakage of nucleic acids and proteins (p < 0.05). Scanning and transmission electron microscopy results showed that US + SAEW treatment could damage ultrastructure of R. stolonifer, resulted in severe cell-wall pitting, completely disrupted into debris, apparent separation of plasma wall, massive vacuoles space, and indistinct intracellular organelles. US500 + SAEW50 treatment at 40 and 55 °C increased cell membrane permeability, and decreased mitochondrial membrane potential of R. stolonifer. In addition, US500 + SAEW50 at 40 °C and US300 + SAEW50 at 55 °C controlled R. stolonifer growth in sweet potato TRs during 20 days of storage, suggesting effective inhibition on the infection of R. stolonifer. Therefore, US + SAEW treatment could be a new efficient alternative method for storing and preserving sweet potato TRs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1350-4177 1873-2828 |
DOI: | 10.1016/j.ultsonch.2021.105528 |