The v-src inducible gene 9E3/pCEF4 is regulated by both its promoter upstream sequence and its 3' untranslated region

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 4; pp. 1162 - 1166
• Main Authors: Blobel, G.A. (The Rockefeller University, New York, NY), Hanafusa, H
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 15.02.1991; National Acad Sciences
• Subjects: ANIMAL EMBRYOS; Animals; ARN DE TRANSFERENCIA; ARN DE TRANSFERT; Base Sequence; Biological and medical sciences; Cell lines; Cells, Cultured; Chick Embryo; CHICKS; Chloramphenicol O-Acetyltransferase - genetics; EMBRIONES ANIMALES; EMBRYON ANIMAL; Enhancer Elements, Genetic; FIBROBLASTE; FIBROBLASTOS; FIBROBLASTS; Fundamental and applied biological sciences. Psychology; GENE; Gene expression; Gene Expression Regulation; GENE MAPPING; GENES; Genes, src; GENETIC REGULATION; GENETIC TRANSFORMATION; GENETICA; GENETICS; GENETIQUE; Messenger RNA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NEOPLASMAS; NEOPLASME; NEOPLASMS; NUCLEOTIDE; NUCLEOTIDES; NUCLEOTIDOS; ONCOGENES; Plasmids; POLLITO; POUSSIN; Promoter regions; Promoter Regions, Genetic; RNA, Messenger - genetics; ROUS SARCOMA VIRUS; Three prime untranslated regions; TRANSCRIPTION; Transcription, Genetic; Transfection; TRANSFER RNA; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; Transformed cell line; Untranslated regions; VIRUS DEL SARCOMA DE ROUS; VIRUS SARCOME DE ROUS; Oncovirinae; Embryo; Upstream sequence; Induction; Transcription promoter; Transformed cell; Retroviridae; Rous sarcoma virus; Gene expression; Virus; Vertebrata; Reporter gene; Transfection; Type C oncovirus; Chicken; Aves; Molecular cloning; Mechanism of action; Fibroblast; Vector
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.4.1162

The 9E3/pCEF4 mRNA is strongly induced in Rous sarcoma virus-transformed chicken embryo fibroblasts when compared to untransformed cells. To identify cis-acting transcriptional elements that confer inducibility by v-src, we isolated the 9E3 promoter upstream region. We found that 1.53 kilobases upstream of the transcriptional start site, when placed in front of a reporter gene, conferred a small degree of inducibility by v-src, in both transient and stable transfections. Two potential AP-1 sites were identified in the 9E3 promoter. AP-1 elements have been implicated previously in mediating a transcriptional response to v-src in fibroblast cell lines. These elements alone do not confer a significant inducibility by v-src in primary chicken embryo fibroblasts. Since the 9E3 mRNA is stabilized in transformed cells, we replaced the 3' untranslated region of the reporter gene with the 9E3 3' untranslated region and found this construct to be strongly responsive to stimulation by v-src. In addition, the 9E3 3' untranslated region increased the response to serum and the tumor promoter phorbol 12-myristate 13-acetate. This suggests that a posttranscriptional mechanism plays a major role in the induction of 9E3 expression.