In yeast, RAS proteins are controlling elements of adenylate cyclase
S. cerevisiae strains containing RAS2 val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutatio...
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Published in | Cell Vol. 40; no. 1; pp. 27 - 36 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge, MA
Elsevier Inc
1985
Cell Press |
Subjects | |
Online Access | Get full text |
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Summary: | S. cerevisiae strains containing
RAS2
val19, a
RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying
IAC or
bcy1. Yeast strains carrying the
IAC mutation have elevated levels of adenylate cyclase activity.
bcy1 is a mutation that suppresses the lethality in adenylate cyclase deficient yeast. Yeast strains deficient in
RAS function exhibit properties similar to adenylate cyclase deficient yeast.
bcy1 suppresses lethality in
ras1
−
ras2
− yeast. Compared to wild-type yeast strains, intracellular cyclic AMP levels are significantly elevated in
RAS2
val19 strains, significantly depressed in
ras2
− strains, and virtually undetectable in
ras1
−
ras2
−
bcy1 strains. Membranes from
ras1
−
ras2
−
bcy1 yeast lack the GTP-stimulated adenylate cyclase activity present in membranes from wild-type cells, and membranes from
RAS2
val19 yeast strains have elevated levels of an apparently GTP-independent adenylate cyclase activity. Mixing membranes from
ras1
−
ras2
− yeast with membranes from adenylate cyclase deficient yeast reconstitutes a GTP-dependent adenylate cyclase. |
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Bibliography: | F30 F ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0092-8674 1097-4172 |
DOI: | 10.1016/0092-8674(85)90305-8 |