Molecularly imprinted polypyrrole based sensor for the detection of SARS-CoV-2 spike glycoprotein

• Published in: Electrochimica acta Vol. 403; p. 139581
• Main Authors: Ratautaite, Vilma, Boguzaite, Raimonda, Brazys, Ernestas, Ramanaviciene, Almira, Ciplys, Evaldas, Juozapaitis, Mindaugas, Slibinskas, Rimantas, Bechelany, Mikhael, Ramanavicius, Arunas
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 20.01.2022; Elsevier BV; Elsevier
• Subjects: Analytical chemistry; Bioengineering; Chemical Sciences; Chemical sensors; Conducting polymers; Coronaviruses; COVID-19; Electrical measurement; Electrochemical determination of virus proteins; Glycoproteins; Imprinted polymers; Life Sciences; Microbiology and Parasitology; Molecular imprinting; Molecularly imprinted polymers (MIPs); Polymers; Polypyrrole (Ppy); Polypyrroles; Proteins; SARS-CoV-2 spike glycoprotein; Sensors; Serum albumin; Severe acute respiratory syndrome coronavirus 2; Thin layers; Viral diseases; Virology; COVID-19; Conducting polymers; Electrochemical determination of virus proteins; SARS-CoV-2 spike glycoprotein; Molecularly imprinted polymers (MIPs); Thin layers; Polypyrrole (Ppy); SARS-CoV-2 Spike glycoprotein; conducting polymers; thin layers; molecularly imprinted polymers (MIPs); electrochemical determination of virus proteins
• ISSN: 0013-4686; 1873-3859; 0013-4686
• DOI: 10.1016/j.electacta.2021.139581

•Molecularly imprinted polypyrrole (MIP-Ppy) based electrochemical sensor is reported.•SARS-CoV-2-S spike glycoprotein was imprinted within Ppy in order to design MIP-Ppy.•Pulsed amperometric detection (PAD) was applied as electroanalytical method.•Complex evaluation of PAD-based electroanalytical signal was performed.•A selectivity of MIP-Ppy-based sensor was determined. This study describes the application of a polypyrrole-based sensor for the determination of SARS-CoV-2-S spike glycoprotein. The SARS-CoV-2-S spike glycoprotein is a spike protein of the coronavirus SARS-CoV-2 that recently caused the worldwide spread of COVID-19 disease. This study is dedicated to the development of an electrochemical determination method based on the application of molecularly imprinted polymer technology. The electrochemical sensor was designed by molecular imprinting of polypyrrole (Ppy) with SARS-CoV-2-S spike glycoprotein (MIP-Ppy). The electrochemical sensors with MIP-Ppy and with polypyrrole without imprints (NIP-Ppy) layers were electrochemically deposited on a platinum electrode surface by a sequence of potential pulses. The performance of polymer layers was evaluated by pulsed amperometric detection. According to the obtained results, a sensor based on MIP-Ppy is more sensitive to the SARS-CoV-2-S spike glycoprotein than a sensor based on NIP-Ppy. Also, the results demonstrate that the MIP-Ppy layer is more selectively interacting with SARS-CoV-2-S glycoprotein than with bovine serum albumin. This proves that molecularly imprinted MIP-Ppy-based sensors can be applied for the detection of SARS-CoV-2 virus proteins. [Display omitted]