Bta-miR-33a affects gene expression and lipid levels in Chinese Holstein mammary epithelial cells
MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19–25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chines...
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| Published in | Archiv für Tierzucht Vol. 65; no. 4; pp. 357 - 370 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Gottingen
Copernicus GmbH
10.10.2022
Copernicus Publications |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2363-9822 0003-9438 2363-9822 |
| DOI | 10.5194/aab-65-357-2022 |
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| Abstract | MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about
19–25 nucleotides in length that regulate different biological processes,
including lipid metabolism. In this study, we explored the effect of
bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs)
of Chinese Holstein for the first time. For this purpose, the plasmids of
bta-miR-33a mimic, bta-miR-33a inhibitor and bta-miR-33a negative control
were constructed to overexpress or repress bta-miR-33a in BMECs. The effects of
plasmid transfection were analysed by examining the mRNA and protein
expression levels of ELOVL6 and the intracellular triglycerides. The results
showed that bta-miR-33a directly inhibited the expression of ELOVL6 in BMECs;
decreased the mRNA levels of ELOVL5, HACD2, CPT1A and MSMO1; and increased the mRNA level of ALOX15.
Sequence bta-miR-33a also increased the contents of triglycerides in the cells,
presumably as a consequence of these gene expression changes. In summary,
the results of the present study suggest that bta-miR-33a regulates lipid
metabolism by targeting ELOVL6, which might be a potential molecular marker of
milk fat composition. |
|---|---|
| AbstractList | MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19–25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chinese Holstein for the first time. For this purpose, the plasmids of bta-miR-33a mimic, bta-miR-33a inhibitor and bta-miR-33a negative control were constructed to overexpress or repress bta-miR-33a in BMECs. The effects of plasmid transfection were analysed by examining the mRNA and protein expression levels of ELOVL6 and the intracellular triglycerides. The results showed that bta-miR-33a directly inhibited the expression of ELOVL6 in BMECs; decreased the mRNA levels of ELOVL5, HACD2, CPT1A and MSMO1; and increased the mRNA level of ALOX15. Sequence bta-miR-33a also increased the contents of triglycerides in the cells, presumably as a consequence of these gene expression changes. In summary, the results of the present study suggest that bta-miR-33a regulates lipid metabolism by targeting ELOVL6, which might be a potential molecular marker of milk fat composition. MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19–25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chinese Holstein for the first time. For this purpose, the plasmids of bta-miR-33a mimic, bta-miR-33a inhibitor and bta-miR-33a negative control were constructed to overexpress or repress bta-miR-33a in BMECs. The effects of plasmid transfection were analysed by examining the mRNA and protein expression levels of ELOVL6 and the intracellular triglycerides. The results showed that bta-miR-33a directly inhibited the expression of ELOVL6 in BMECs; decreased the mRNA levels of ELOVL5, HACD2, CPT1A and MSMO1; and increased the mRNA level of ALOX15. Sequence bta-miR-33a also increased the contents of triglycerides in the cells, presumably as a consequence of these gene expression changes. In summary, the results of the present study suggest that bta-miR-33a regulates lipid metabolism by targeting ELOVL6, which might be a potential molecular marker of milk fat composition. MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19-25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chinese Holstein for the first time. For this purpose, the plasmids of bta-miR-33a mimic, bta-miR-33a inhibitor and bta-miR-33a negative control were constructed to overexpress or repress bta-miR-33a in BMECs. The effects of plasmid transfection were analysed by examining the mRNA and protein expression levels of ELOVL6 and the intracellular triglycerides. The results showed that bta-miR-33a directly inhibited the expression of ELOVL6 in BMECs; decreased the mRNA levels of ELOVL5, HACD2, CPT1A and MSMO1; and increased the mRNA level of ALOX15. Sequence bta-miR-33a also increased the contents of triglycerides in the cells, presumably as a consequence of these gene expression changes. In summary, the results of the present study suggest that bta-miR-33a regulates lipid metabolism by targeting ELOVL6, which might be a potential molecular marker of milk fat composition.MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19-25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chinese Holstein for the first time. For this purpose, the plasmids of bta-miR-33a mimic, bta-miR-33a inhibitor and bta-miR-33a negative control were constructed to overexpress or repress bta-miR-33a in BMECs. The effects of plasmid transfection were analysed by examining the mRNA and protein expression levels of ELOVL6 and the intracellular triglycerides. The results showed that bta-miR-33a directly inhibited the expression of ELOVL6 in BMECs; decreased the mRNA levels of ELOVL5, HACD2, CPT1A and MSMO1; and increased the mRNA level of ALOX15. Sequence bta-miR-33a also increased the contents of triglycerides in the cells, presumably as a consequence of these gene expression changes. In summary, the results of the present study suggest that bta-miR-33a regulates lipid metabolism by targeting ELOVL6, which might be a potential molecular marker of milk fat composition. MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19–25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chinese Holstein for the first time. For this purpose, the plasmids of bta-miR-33a mimic, bta-miR-33a inhibitor and bta-miR-33a negative control were constructed to overexpress or repress bta-miR-33a in BMECs. The effects of plasmid transfection were analysed by examining the mRNA and protein expression levels of ELOVL6 and the intracellular triglycerides. The results showed that bta-miR-33a directly inhibited the expression of ELOVL6 in BMECs; decreased the mRNA levels of ELOVL5 , HACD2 , CPT1A and MSMO1 ; and increased the mRNA level of ALOX15 . Sequence bta-miR-33a also increased the contents of triglycerides in the cells, presumably as a consequence of these gene expression changes. In summary, the results of the present study suggest that bta-miR-33a regulates lipid metabolism by targeting ELOVL6 , which might be a potential molecular marker of milk fat composition. MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19–25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chinese Holstein for the first time. For this purpose, the plasmids of bta-miR-33a mimic, bta-miR-33a inhibitor and bta-miR-33a negative control were constructed to overexpress or repress bta-miR-33a in BMECs. The effects of plasmid transfection were analysed by examining the mRNA and protein expression levels of ELOVL6 and the intracellular triglycerides. The results showed that bta-miR-33a directly inhibited the expression of ELOVL6 in BMECs; decreased the mRNA levels of ELOVL5, HACD2, CPT1A and MSMO1; and increased the mRNA level of ALOX15. Sequence bta-miR-33a also increased the contents of triglycerides in the cells, presumably as a consequence of these gene expression changes. In summary, the results of the present study suggest that bta-miR-33a regulates lipid metabolism by targeting ELOVL6, which might be a potential molecular marker of milk fat composition. |
| Audience | Academic |
| Author | Iqbal, Ambreen Yu, Haibin Jiang, Ping Cui, Zhiqian Zhao, Zhihui |
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| Copyright | COPYRIGHT 2022 Copernicus GmbH 2022. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Copyright: © 2022 Ping Jiang et al. Copyright: © 2022 Ping Jiang et al. 2022 |
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| Snippet | MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about
19–25 nucleotides in length that regulate different biological processes,
including... MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19-25 nucleotides in length that regulate different biological processes, including... MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19–25 nucleotides in length that regulate different biological processes, including... |
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| SubjectTerms | Animal fat Binding sites Biological activity Body fat Cholesterol Epithelial cells Epithelium Fatty acids Gene expression Genes Genetic research Insulin Kinases Laboratory animals Lipid metabolism Lipids Mammary gland Metabolism MicroRNAs Milk miRNA Nucleotides Oils & fats Original Study Oxidation Physiological aspects Plasmids Proteins RNA Roles Sterols Transcription factors Transfection Triglycerides |
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| Title | Bta-miR-33a affects gene expression and lipid levels in Chinese Holstein mammary epithelial cells |
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