Bta-miR-33a affects gene expression and lipid levels in Chinese Holstein mammary epithelial cells

MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19–25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chines...

Full description

Saved in:
Bibliographic Details
Published inArchiv für Tierzucht Vol. 65; no. 4; pp. 357 - 370
Main Authors Jiang, Ping, Iqbal, Ambreen, Cui, Zhiqian, Yu, Haibin, Zhao, Zhihui
Format Journal Article
LanguageEnglish
Published Gottingen Copernicus GmbH 10.10.2022
Copernicus Publications
Subjects
Animal fat
Binding sites
Biological activity
Body fat
Cholesterol
Epithelial cells
Epithelium
Fatty acids
Gene expression
Genes
Genetic research
Insulin
Kinases
Laboratory animals
Lipid metabolism
Lipids
Mammary gland
Metabolism
MicroRNAs
Milk
miRNA
Nucleotides
Oils & fats
Original Study
Oxidation
Physiological aspects
Plasmids
Proteins
RNA
Roles
Sterols
Transcription factors
Transfection
Triglycerides
China
Online AccessGet full text

Cover

More Information
Summary:MicroRNAs (miRNAs) are non-coding single-stranded RNA molecules of about 19–25 nucleotides in length that regulate different biological processes, including lipid metabolism. In this study, we explored the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs) of Chinese Holstein for the first time. For this purpose, the plasmids of bta-miR-33a mimic, bta-miR-33a inhibitor and bta-miR-33a negative control were constructed to overexpress or repress bta-miR-33a in BMECs. The effects of plasmid transfection were analysed by examining the mRNA and protein expression levels of ELOVL6 and the intracellular triglycerides. The results showed that bta-miR-33a directly inhibited the expression of ELOVL6 in BMECs; decreased the mRNA levels of ELOVL5, HACD2, CPT1A and MSMO1; and increased the mRNA level of ALOX15. Sequence bta-miR-33a also increased the contents of triglycerides in the cells, presumably as a consequence of these gene expression changes. In summary, the results of the present study suggest that bta-miR-33a regulates lipid metabolism by targeting ELOVL6, which might be a potential molecular marker of milk fat composition.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2363-9822
0003-9438
2363-9822
DOI:10.5194/aab-65-357-2022