Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay

Oral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (P. gingivalis, T. forsythia, T. denticola, F. nucleatum, C. rectus, P. intermedia, A. actinomycete...

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Published inJournal of clinical medicine Vol. 9; no. 9; p. 2945
Main Authors Paqué, Pune N., Herz, Christopher, Jenzer, Joël S., Wiedemeier, Daniel B., Attin, Thomas, Bostanci, Nagihan, Belibasakis, Georgios N., Bao, Kai, Körner, Philipp, Fritz, Tanja, Prinz, Julia, Schmidlin, Patrick R., Thurnheer, Thomas, Wegehaupt, Florian J., Mitsakakis, Konstantinos, Peham, Johannes R.
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 11.09.2020
MDPI
Subjects
Antibiotics
Clinical medicine
Dental caries
Dentistry
Dentists
Diabetes
Gum disease
Infections
Medicin och hälsovetenskap
Medicine
Microbiota
Oral diseases
Oral hygiene
Patients
Primary care
Switzerland
Germany
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Summary:Oral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (P. gingivalis, T. forsythia, T. denticola, F. nucleatum, C. rectus, P. intermedia, A. actinomycetemcomitans, S. mutans, S. sobrinus, oral associated Lactobacilli) in saliva and their clinical status association in 214 individuals. Upon clinical examination, study subjects were grouped into healthy, caries and periodontitis and their saliva was collected. A highly specific point-of-care compatible dual color qPCR assay was developed and used to study the above-mentioned bacteria of interest in the collected saliva. Assay performance was compared to a commercially available microbial reference test. Eight out of ten taxa that were investigated during this study were strong discriminators between the periodontitis and healthy groups: C. rectus, T. forsythia, P. gingivalis, S. mutans, F. nucleatum, T. denticola, P. intermedia and oral Lactobacilli (p < 0.05). Significant differentiation between the periodontitis and caries group microbiome was only shown for S. mutans (p < 0.05). A clear distinction between oral health and disease was enabled by the analysis of quantitative qPCR data of target taxa levels in saliva.
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Shared co-first authorship: These authors contributed equally to this work.
ISSN:2077-0383
2077-0383
DOI:10.3390/jcm9092945