ENZYME IMMUNOASSAY OF CYCLAZOCINE USING PEROXIDASE AS LABEL

A sensitive enzyme immunoassay for the determination of serum or urinary cyclazocine was developed. Horseradish peroxidase was used as the labelling enzyme ; it was conjugated with cyclazocine derivatives by the mixed anhydride method. After the immune reaction, bound and free fractions were separat...

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Published inJournal of Pharmacobio-Dynamics Vol. 5; no. 6; pp. 373 - 378
Main Authors KOSUGE, HIROSHI, MAEDA, MASAKO, TSUJI, AKIO
Format Journal Article
LanguageEnglish
Published Japan The Pharmaceutical Society of Japan 01.01.1982
Subjects
Cyclazocine - blood
cyclazocine-HRP conjugate
Horseradish Peroxidase
Humans
Hydrogen Peroxide - metabolism
Immunoenzyme Techniques
Phenylpropionates - metabolism
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Summary:A sensitive enzyme immunoassay for the determination of serum or urinary cyclazocine was developed. Horseradish peroxidase was used as the labelling enzyme ; it was conjugated with cyclazocine derivatives by the mixed anhydride method. After the immune reaction, bound and free fractions were separated by a double-antibody solid-phase method, using Sepharose 4B gel coupled with purified IgG from goat anti-rabbit IgG serum. The enzyme activity was measured fluorophotometrically, with p-hydroxyphenyl propionic acid and hydrogen peroxide as substrates. The correlation coefficients verified that there was excellent agreement between the results obtained by the new enzyme immunoassay and those of radioimmunoassay (r=0.92) and gas chromatography-mas spectrometry (r=0.94).
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ISSN:0386-846X
1881-1353
DOI:10.1248/bpb1978.5.373