Development of a Rapid, Reliable Genetic Test for Pseudoxanthoma Elasticum

• Published in: The Journal of molecular diagnostics : JMD Vol. 9; no. 1; pp. 105 - 112
• Main Authors: Shi, Yanggu, Terry, Sharon F, Terry, Patrick F, Bercovitch, Lionel G, Gerard, Gary F
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2007; ASIP; American Society for Investigative Pathology
• Subjects: Diagnosis, Differential; Electrophoresis, Agar Gel; Exons - genetics; Humans; Molecular Diagnostic Techniques - methods; Multidrug Resistance-Associated Proteins - genetics; Pathology; Point Mutation - genetics; Polymerase Chain Reaction - methods; Pseudoxanthoma Elasticum - genetics; Regular; Sequence Analysis, DNA
• ISSN: 1525-1578; 1943-7811
• DOI: 10.2353/jmoldx.2007.060093

Mutations in the human ABCC6 gene cause pseudoxanthoma elasticum (PXE), a hereditary disorder that impacts the skin, eyes, and cardiovascular system. Currently, the diagnosis of PXE is based on physical findings and histological examination of a biopsy of affected skin. We have combined two simple, polymerase chain reaction (PCR)-based methods to develop a rapid, reliable genetic assay for the majority of known PXE mutations. After PCR amplification and heteroduplex formation, mutations in exon 24 and exon 28 of the ABCC6 gene were detected with Surveyor nuclease, which cleaves double-stranded DNA at any mismatch site. Mutations originating from deletion of a segment of the ABCC6 gene between exon 23 and exon 29 (ex23_ex29del) were detected by long-range PCR. Size analysis of digestion fragments and long-range PCR products was performed by agarose gel electrophoresis. The methods accurately identified mutations or the absence thereof in 16 affected individuals as confirmed by DNA sequencing. Fifteen patients had one or two point mutations, and two of these individuals carried the ex23_ex29del in their second allele. This mutation detection and mapping strategy provides a simple and reliable genetic assay to assist in diagnosis of PXE, differential diagnosis of PXE-like conditions, and study of PXE genetics.