Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode

• Published in: Sensors (Basel, Switzerland) Vol. 23; no. 6; p. 3209
• Main Authors: Li, Yang-Yang, Ma, Xin-Xin, Song, Xin-Yan, Ma, Lin-Lin, Li, Yu-Ying, Meng, Xin, Chen, Yu-Jie, Xu, Ke-Xin, Moosavi-Movahedi, Ali Akbar, Xiao, Bao-Lin, Hong, Jun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.03.2023; MDPI
• Subjects: Biocompatibility; Biosensing Techniques - methods; biosensor; Biosensors; Biotechnology; Blood sugar; bovine serum albumin; Carbon; Chronic diseases; Crosslinking; Detectors; Diabetes; Diabetes therapy; Electrochemical Techniques - methods; Electrodes; Enzymes; Enzymes, Immobilized - chemistry; Glassy carbon; Glucose; Glucose - chemistry; Glucose oxidase; Glucose Oxidase - chemistry; High performance liquid chromatography; Hydrophobicity; hydroxyl fullerenes; multi-walled carbon nanotubes; Nanocomposites; Nanocomposites - chemistry; Nanoparticles; Nanotubes, Carbon - chemistry; Plasma; Reagents; Serum albumin; Serum Albumin, Bovine - chemistry; Stability analysis; Storage stability; Canada; China; Beijing China; United States > US; biosensor; hydroxyl fullerenes; bovine serum albumin; glucose oxidase; multi-walled carbon nanotubes
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s23063209

Glucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modified by a composite of hydroxy fullerene (HFs) and multi-walled carbon nanotubes (MWCNTs) and protected with a glutaraldehyde (GLA)/Nafion (NF) composite membrane to prepare a novel glucose biosensor. The modified materials were analyzed by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry (CV). The prepared MWCNTs-HFs composite has excellent conductivity, the addition of BSA regulates MWCNTs-HFs hydrophobicity and biocompatibility, and better immobilizes GOD on MWCNTs-HFs. MWCNTs-BSA-HFs plays a synergistic role in the electrochemical response to glucose. The biosensor shows high sensitivity (167 μA·mM ·cm ), wide calibration range (0.01-3.5 mM), and low detection limit (17 μM). The apparent Michaelis-Menten constant Kmapp is 119 μM. Additionally, the proposed biosensor has good selectivity and excellent storage stability (120 days). The practicability of the biosensor was evaluated in real plasma samples, and the recovery rate was satisfactory.