Evolution, antimicrobial susceptibility and assignment to international clones of methicillin-resistant Staphylococcus aureus isolated over a 9-year period in two Spanish hospitals

• Published in: Clinical microbiology and infection Vol. 13; no. 7; pp. 728 - 730
• Main Authors: Potel, C., Álvarez, M., Álvarez, P., Otero, I., Fluiters, E.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.07.2007; Blackwell Publishing Ltd; Blackwell
• Subjects: Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial diseases; Bacterial Typing Techniques; Biological and medical sciences; Brazil - epidemiology; Cross Infection - epidemiology; Cross Infection - microbiology; Disease Outbreaks; Drug Resistance, Bacterial; Epidemic clones; Evolution, Molecular; Human bacterial diseases; Humans; identification; Infectious diseases; Japan - epidemiology; Medical sciences; Methicillin Resistance - genetics; Microbial Sensitivity Tests; molecular analysis; MRSA; New York - epidemiology; Pharmacology. Drug treatments; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; Sequence Analysis, DNA; Spain; Spain - epidemiology; Staphylococcal Infections - epidemiology; Staphylococcal Infections - microbiology; Staphylococcal infections, streptococcal infections, pneumococcal infections; Staphylococcus aureus; Staphylococcus aureus - classification; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; typing; New York; Asia; United States; Europe; Japan; America; North America; Spain; Brazil; typing; identification; molecular analysis; Epidemic clones; MRSA; Spain; Drug susceptibility test; Identification; Meticillin; Penicillin derivatives; Microbiological investigation; Bacteria; Micrococcales; Evolution; Micrococcaceae; Hospital; Staphylococcus aureus; Epidemic; Typing; β-Lactams; Infection; Polymerase chain reaction; Antibiotic; Bacteriosis; Molecular biology; Antibacterial agent; Staphylococcal infection
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2007.01728.x

Antimicrobial resistance profiles, restriction fragment length polymorphism of the coagulase gene and repetitive element sequence-based PCR were used to classify 210 methicillin-resistant Staphylococcus aureus isolates recovered between 1997 and 2005 in two hospitals in Vigo, north-west Spain. Representative isolates belonging to the epidemic clones were analysed by spa typing and multilocus sequence typing, and the staphylococcal chromosomal cassette (SCC)mec type was determined for all isolates. The New York/Japan clone (t002-ST5-II) was detected in Spain for the first time. However, the New York/Japan and the Brazilian (t037-ST239-IIIA) clones were replaced by EMRSA-16 (t018-ST36-II), which at present is the predominant clone.