Increased Methylglyoxal and Oxidative Stress in Hypertensive Rat Vascular Smooth Muscle Cells

• Published in: Hypertension (Dallas, Tex. 1979) Vol. 39; no. 3; pp. 809 - 814
• Main Authors: Wu, Lingyun, Juurlink, Bernhard H.J
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Heart Association, Inc 01.03.2002; Hagerstown, MD Lippincott
• Subjects: Animals; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cells, Cultured; Experimental diseases; Fluoresceins - metabolism; Glutathione - metabolism; Glutathione Peroxidase - metabolism; Glutathione Reductase - metabolism; Glutathione Transferase - metabolism; Glycation End Products, Advanced - metabolism; Hypertension - metabolism; Intercellular Adhesion Molecule-1 - metabolism; Medical sciences; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - metabolism; NF-kappa B - metabolism; Oxidation-Reduction; Oxidative Stress; Pyruvaldehyde - metabolism; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Species Specificity; Hypertension; Oxidative stress; Vertebrata; Mammalia; Rat; Pathogenesis; Animal; Blood vessel; Rodentia; Cardiovascular disease; Smooth muscle; Pyruvaldehyde
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/hy0302.105207

Methylglyoxal can yield advanced glycation end products via nonenzymatic glycation of proteins. Whether methylglyoxal contributes to the pathogenesis of hypertension has not been clear. The aim of the present study was to investigate whether the levels of methylglyoxal and methylglyoxal-induced advanced glycation end products were enhanced and whether methylglyoxal increased oxidative stress, activated nuclear factor–κB (NF-κB), and increased intracellular adhesion molecule-1 (ICAM-1) content in vascular smooth muscle cells from spontaneously hypertensive rats. Basal cellular levels of methylglyoxal and advanced glycation end products were more than 2-fold higher (P <0.05) in cells from hypertensive rats than from normotensive Wistar-Kyoto rats. This correlated with levels of oxidative stress and oxidized glutathione that were significantly higher in cells from hypertensive rats, whereas levels of glutathione and activities of glutathione reductase and glutathione peroxidase were significantly lower. Basal levels of nuclearly localized NF-κB p65 and ICAM-1 protein expression were higher in cells from hypertensive rats than from normotensive rats. Addition of exogenous methylglyoxal to the cultures induced a greater increase in oxidative stress and advanced glycation end products in cells from hypertensive rats compared with normotensive rats and significantly decreased the activities of glutathione reductase and glutathione peroxidase in cells of both rat strains. Methylglyoxal activated NF-κB p65 and increased ICAM-1 expression in hypertensive cells, which was inhibited by N-acetylcysteine. Our study demonstrates an elevated methylglyoxal level and advanced glycation end products in cells from hypertensive rats, and methylglyoxal increases oxidative stress, activates NF-κB, and enhances ICAM-1 expression. Our findings suggest that that elevated methylglyoxal and associated oxidative stress possibly contribute to the pathogenesis of hypertension.