Polyphosphate kinase: a new colonization factor of Helicobacter pylori

• Published in: FEMS microbiology letters Vol. 243; no. 1; pp. 45 - 50
• Main Authors: Ayraud, Sarah, Janvier, Blandine, Labigne, Agnès, Ecobichon, Chantal, Burucoa, Christophe, Fauchère, Jean-Louis
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.02.2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Animals; Bacteriology; Biological and medical sciences; Colonization; Colonization factor; Deficient mutant; Experimental infection; Female; Fundamental and applied biological sciences. Psychology; Gastric mucosa; Gastric Mucosa - microbiology; Helicobacter Infections - microbiology; Helicobacter pylori; Helicobacter pylori - enzymology; Helicobacter pylori - growth & development; Helicobacter pylori - isolation & purification; Helicobacter pylori - pathogenicity; Inoculation; Kinases; Metabolism. Enzymes; Mice; Mice, Inbred C57BL; Microbiology; Mutation; Phosphotransferases (Phosphate Group Acceptor) - metabolism; Polyphosphate kinase; Strains (organisms); Virulence; Colonization factor; Experimental infection; Polyphosphate kinase; Helicobacter pylori; Statistical analysis; Spirillales; Enzyme; Transferases; Spirillaceae; Infection; Transmission electron microscopy; Bacteria; Reverse transcription polymerase chain reaction; Colonization
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/j.femsle.2004.11.040

In order to elucidate the role of polyphosphate kinase (PPK) during the course of an infection by Helicobacter pylori, PPK deficient mutants were constructed using two genetic backgrounds: Hp141v and X47-2AL. The efficiencies of the parental strains and the derivative mutants at colonizing the gastric mucosa of mice were compared. When animals received the Hp141v and the X47-2AL parental strains, 100% of the mice remained colonized for the duration of the 45 days experiment. In contrast, none of the mice that were given the PPK deficient X47-2AL derivative strain had a detectable bacterial load in their gastric mucosa, while the deficient Hp141v derivative strain was detected in 100%, 20% and 40% of the mice at days 3, 15 and 45 post-inoculation (p.i.), respectively. The absence of PPK expression did not impair the in vitro growth of the ppk mutants. However, the reduced ability of the ppk defective mutants to colonize mice was associated with a significant decrease in both motility and in an accumulation of polyP in the bacterial cells. These results are consistent with an essential role of PPK during the initial steps of colonisation of the mouse gastric mucosa and confirm that PPK may act on the virulence of H. pylori partly through an energy dependent mechanism.