Evaluation of the use of RT-PCR for the early diagnosis of dengue fever

RT-PCR was used to diagnose dengue virus infections confirmed serologically in 26 returning travellers. RT-PCR was positive for three (75%) of four samples taken on or before day 3 of the illness, for 15 (78.9%) of 19 samples taken between days 4 and 7, and for none of three samples tested on or aft...

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Published inClinical microbiology and infection Vol. 12; no. 4; pp. 395 - 397
Main Authors Grobusch, M.P., Niedrig, M., Göbels, K., Klipstein-Grobusch, K., Teichmann, D.
Format Journal Article
LanguageEnglish
Published Oxford, UK Elsevier Ltd 01.04.2006
Blackwell Publishing Ltd
Blackwell
Subjects
Adolescent
Adult
Arboviroses
Biological and medical sciences
Dengue - diagnosis
Dengue fever
Dengue fevers
Dengue virus
Dengue Virus - genetics
Dengue Virus - isolation & purification
diagnosis
Early Diagnosis
Female
Human viral diseases
Humans
Infectious diseases
Male
Medical sciences
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - analysis
RT-PCR
sampling time
sensitivity
Tropical viral diseases
Viral diseases
diagnosis
sensitivity
Dengue fever
RT-PCR
sampling time
Infection
Viral disease
Dengue
Arbovirus disease
Diagnosis
Molecular biology
Reverse transcription polymerase chain reaction
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Summary:RT-PCR was used to diagnose dengue virus infections confirmed serologically in 26 returning travellers. RT-PCR was positive for three (75%) of four samples taken on or before day 3 of the illness, for 15 (78.9%) of 19 samples taken between days 4 and 7, and for none of three samples tested on or after day 8 (p 0.0337). When applied early, RT-PCR seems to be a useful tool for the diagnosis of dengue fever.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1198-743X
1469-0691
DOI:10.1111/j.1469-0691.2006.01353.x