Survivability and life support in sealed mini-ecosystems with simulated planetary soils

• Published in: Scientific reports Vol. 14; no. 1; pp. 26322 - 15
• Main Authors: Sato, Tsubasa, Abe, Ko, Koseki, Jun, Seto, Mayumi, Yokoyama, Jun, Akashi, Tomohiro, Terada, Masahiro, Kadowaki, Kohmei, Yoshida, Satoshi, Yamashiki, Yosuke Alexandre, Shimamura, Teppei
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.11.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/158; 631/337; Animals; Biosphere; Cultivation; Cyanobacteria - growth & development; Cyanobacteria - metabolism; Cyanobacteria - physiology; Ecological Systems, Closed; Ecosystem; Ecosystem stability; Ecosystems; Extraterrestrial Environment; Fruit cultivation; Groundwater; Groundwater - microbiology; Humanities and Social Sciences; Life Support Systems; Light sources; Metagenomics; Microbial activity; Microbiomes; Microbiota; Moisture deficiency; multidisciplinary; Plant growth; Plants - metabolism; Plants - microbiology; Science; Science (multidisciplinary); Soil - chemistry; Soil Microbiology; Space Flight; Terrestrial ecosystems
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-75328-x

Establishing a sustainable life-support system for space exploration is a formidable challenge due to the vast distances, high costs, and environmental differences from Earth. Building upon the lessons from the Biosphere 2 experiment, we introduce the novel “Ecosphere” and “Biosealed” systems, self-sustaining ecosystems within customizable, enclosed containers. These systems incorporate terrestrial ecosystems and groundwater layers, offering a potential model for transplanting Earth-like biomes to extraterrestrial environments. Over 4 years, we conducted rigorous experiments and analyses to understand the dynamics of these enclosed ecosystems. We successfully mitigated moisture deficiency, a major obstacle to plant growth, by incorporating groundwater layers. Additionally, we quantified microbial communities proliferating in specific soils, including simulated lunar and Ryugu asteroid regolith, enhance plant cultivation in space environments. Metagenomic analysis of these simulated space soils revealed diverse microbial populations and their crucial role in plant growth and ecosystem stability. Notably, we identified symbiotic relationships between plants and Cyanobacteria , enhancing oxygen production, and demonstrated the potential of LED lighting as an alternative light source for plant cultivation in sun-limited space missions. We also confirmed the survival of fruit flies within these systems, relying on plant-produced oxygen and photosynthetic bacteria. Our research provides a comprehensive framework for developing future space life-support systems. The novelty of our work lies in the unique design of our enclosed ecosystems, incorporating groundwater layers and simulated extraterrestrial soils, and the detailed analysis of microbial communities within these systems. These findings offer valuable insights into the challenges and potential solutions for establishing sustainable human habitats in space, including the importance of microbial management and potential health concerns related to microbial exposure.