Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture
Six endophytic bacteria of corn roots were identified as Bacillus sp. and as Enterobacter sp, by sequencing of the 16S rRNA gene. Four of the strains, CNPSo 2476, CNPSo 2477, CNPSo 2478 and CNPSo 2480 were positive for the nitrogen fixation ability evaluated through the acetylene reduction assay and...
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Published in | AMB Express Vol. 4; no. 1; p. 26 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
07.05.2014
Springer Nature B.V Springer |
Subjects | |
Online Access | Get full text |
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Summary: | Six endophytic bacteria of corn roots were identified as
Bacillus
sp. and as
Enterobacter
sp, by sequencing of the 16S rRNA gene. Four of the strains, CNPSo 2476, CNPSo 2477, CNPSo 2478 and CNPSo 2480 were positive for the nitrogen fixation ability evaluated through the acetylene reduction assay and amplification of
nifH
gene. Two
Bacillus
strains (CNPSo 2477 and CNPSo 2478) showed outstanding skills for the production of IAA, siderophores and lytic enzymes, but were not good candidates as growth promoters, because they reduced seed germination. However, the same strains were antagonists against the pathogenic fungi
Fusarium verticillioides
,
Colletotrichum graminicola
,
Bipolaris maydis
and
Cercospora zea-maydis
. As an indication of favorable bacterial action,
Enterobacter
sp. CNPSo 2480 and
Bacillus
sp. CNPSo 2481 increased the root volume by 44% and 39%, respectively, and the seed germination by 47% and 56%, respectively. Therefore, these two strains are good candidates for future testing as biological inoculants for corn. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2191-0855 2191-0855 |
DOI: | 10.1186/s13568-014-0026-y |