Life cycle progression and sexual development of the apicomplexan parasite Cryptosporidium parvum

• Published in: Nature microbiology Vol. 4; no. 12; pp. 2226 - 2236
• Main Authors: Tandel, Jayesh, English, Elizabeth D., Sateriale, Adam, Gullicksrud, Jodi A., Beiting, Daniel P., Sullivan, Megan C., Pinkston, Brittain, Striepen, Boris
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2019; Nature Publishing Group
• Subjects: 631/326/417/1716; 631/326/417/2549; 631/326/88; Animals; Biomedical and Life Sciences; Cell culture; Children; Cre recombinase; Cryptosporidiosis - parasitology; Cryptosporidium; Cryptosporidium parvum - cytology; Cryptosporidium parvum - genetics; Cryptosporidium parvum - growth & development; Disease Models, Animal; Disease transmission; Female; Fertilization; Food contamination; Gametes; Gene Expression; Genes, Protozoan - genetics; Genetic engineering; Homeodomain Proteins - genetics; Infectious Diseases; Interferon-gamma - genetics; Intestine; Life Cycle Stages - physiology; Life cycles; Life Sciences; Male; Medical Microbiology; Meiosis; Mice; Mice, Knockout; Microbiology; mRNA; Oocysts; Parasites; Parasitology; Sequence Analysis, RNA; Sex; Sexual Development - physiology; Spores; Sporulation; Virology
• ISSN: 2058-5276; 2058-5276
• DOI: 10.1038/s41564-019-0539-x

The apicomplexan parasite Cryptosporidium is a leading global cause of severe diarrhoeal disease and an important contributor to early childhood mortality. Currently, there are no fully effective treatments or vaccines available. Parasite transmission occurs through ingestion of oocysts, through either direct contact or consumption of contaminated water or food. Oocysts are meiotic spores and the product of parasite sex. Cryptosporidium has a single-host life cycle in which both asexual and sexual processes occur in the intestine of infected hosts. Here, we genetically engineered strains of Cryptosporidium to make life cycle progression and parasite sex tractable. We derive reporter strains to follow parasite development in culture and in infected mice and define the genes that orchestrate sex and oocyst formation through mRNA sequencing of sorted cells. After 2 d, parasites in cell culture show pronounced sexualization, but productive fertilization does not occur and infection falters. By contrast, in infected mice, male gametes successfully fertilize female parasites, which leads to meiotic division and sporulation. To rigorously test for fertilization, we devised a two-component genetic-crossing assay using a reporter that is activated by Cre recombinase. Our findings suggest obligate developmental progression towards sex in Cryptosporidium , which has important implications for the treatment and prevention of the infection. Infection with Cryptosporidium parvum is a leading cause of severe diarrhoeal disease and childhood mortality worldwide. Using tools they recently developed to genetically engineer Cryptosporidium , the authors define life cycle stage-specific markers and generate reporter parasites, making life cycle progression and parasite sex tractable.