Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system

Dear Editor, Animals carrying exogenous genes integrated at specific genomic loci are versatile tools for biological research [1]. Zebrafish （Danio rerio）, an emerging vertebrate animal model, is widely used in studies on genetics, developmental biology and neurobiology. Although loss-of-function ge...

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Bibliographic Details
Published inCell research Vol. 25; no. 5; pp. 634 - 637
Main Authors Li, Jia, Zhang, Bai-bing, Ren, Yong-gang, Gu, Shan-ye, Xiang, Yuan-hang, Du, Jiu-lin
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.05.2015
Subjects
Animals
Clustered Regularly Interspaced Short Palindromic Repeats - genetics
Danio rerio
Freshwater
Gene Knock-In Techniques
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Introns - genetics
Letter to the Editor
Zebrafish
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
介导
内含子
性基因
整性
斑马鱼
神经生物学
系统
脊椎动物模型
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Summary:Dear Editor, Animals carrying exogenous genes integrated at specific genomic loci are versatile tools for biological research [1]. Zebrafish （Danio rerio）, an emerging vertebrate animal model, is widely used in studies on genetics, developmental biology and neurobiology. Although loss-of-function genomic editing for zebrafish has been well developed [2-5], lack of feasible methods for inserting a large exogenous DNA sequence into the zebrafish genome is becoming a bottleneck for zebrafish-relevant research.
Bibliography:Dear Editor, Animals carrying exogenous genes integrated at specific genomic loci are versatile tools for biological research [1]. Zebrafish （Danio rerio）, an emerging vertebrate animal model, is widely used in studies on genetics, developmental biology and neurobiology. Although loss-of-function genomic editing for zebrafish has been well developed [2-5], lack of feasible methods for inserting a large exogenous DNA sequence into the zebrafish genome is becoming a bottleneck for zebrafish-relevant research.
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These two authors contributed equally to this work.
ISSN:1001-0602
1748-7838
DOI:10.1038/cr.2015.43