Precision genetic modifications: a new era in molecular biology and crop improvement

Recently, the use of programmable DNA-binding proteins such as ZFP/ZFNs, TALE/TALENs and CRISPR/Cas has produced unprecedented advances in gene targeting and genome editing in prokaryotes and eukaryotes. These advances allow researchers to specifically alter genes, reprogram epigenetic marks, genera...

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Bibliographic Details
Published inPlanta Vol. 239; no. 4; pp. 921 - 939
Main Authors Fichtner, Franziska, Castellanos, Reynel Urrea, Ülker, Bekir
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 01.04.2014
Springer Berlin Heidelberg
Springer Nature B.V
Subjects
Agriculture
Amino Acid Sequence
Base Sequence
Biomedical and Life Sciences
Crop improvement
Crops, Agricultural - genetics
DNA
DNA-Binding Proteins - genetics
Ecology
EMERGING TECHNOLOGIES
Epigenesis, Genetic
Forestry
Gene Targeting - methods
Genes
Genetic engineering
Genetic Engineering - methods
Genetic mutation
Genetic Techniques
Genome, Plant - genetics
Genomes
Life Sciences
Molecular biology
Molecular Sequence Data
Phenotype
Plant Sciences
Plants
Protein Structure, Tertiary
Recombination, Genetic
Rice
RNA
Sequence Alignment
Transgenic plants
Zinc
TALE
CRISPR
ZFN
Gene targeting
Cas9
TALEN
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Summary:Recently, the use of programmable DNA-binding proteins such as ZFP/ZFNs, TALE/TALENs and CRISPR/Cas has produced unprecedented advances in gene targeting and genome editing in prokaryotes and eukaryotes. These advances allow researchers to specifically alter genes, reprogram epigenetic marks, generate site-specific deletions and potentially cure diseases. Unlike previous methods, these precision genetic modification techniques (PGMs) are specific, efficient, easy to use and economical. Here we discuss the capabilities and pitfalls of PGMs and highlight the recent, exciting applications of PGMs in molecular biology and crop genetic engineering. Further improvement of the efficiency and precision of PGM techniques will enable researchers to precisely alter gene expression and biological/chemical pathways, probe gene function, modify epigenetic marks and improve crops by increasing yield, quality and tolerance to limiting biotic and abiotic stress conditions.
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ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-014-2029-y