Sleeve gastrectomy ameliorates alveolar structures and surfactant protein expression in lungs of obese and diabetic rats

• Published in: International Journal of Obesity Vol. 44; no. 12; pp. 2394 - 2404
• Main Authors: Ruze, Rexiati, Li, Jianwen, Xu, Qian, Zhong, Mingwei, Xiong, Yacheng, Yan, Zhibo, Zhu, Jiankang, Cheng, Yugang, Ma, Zhe, Hu, Sanyuan, Zhang, Guangyong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2020; Nature Publishing Group
• Subjects: 101/28; 13/105; 13/106; 14/63; 38/1; 38/79; 631/443/319/1642/393; 692/163/2743/137/138; 82/1; 82/79; 82/80; 96/63; Alveoli; Body weight loss; Care and treatment; Collagen; Complications and side effects; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diffusion barriers; Epidemiology; Gastrectomy; Gastrointestinal surgery; Glucose metabolism; Health Promotion and Disease Prevention; Hydroxyproline; Inflammation; Internal Medicine; Lipids; Lungs; Medicine; Medicine & Public Health; Metabolic Diseases; Obesity; Physiological aspects; Protein expression; Proteins; Public Health; Pulmonary functions; Respiratory function; Respiratory tract diseases; Risk factors; Rodents; Surfactants; Surgery; Triglycerides; Type 2 diabetes; Weight loss; China
• ISSN: 0307-0565; 1476-5497
• DOI: 10.1038/s41366-020-0647-y

Background Bariatric surgeries have been shown to be effective in reversing damaged pulmonary function in individuals suffering from obesity and type 2 diabetes mellitus, whereas its underlying mechanisms remain largely unknown. Methods Sleeve gastrectomy (SG) was performed on obese and diabetic Wistar rats, and their pulmonary function and lung tissues were compared to sham-operated (SH) obese and diabetic rats, and age-matched healthy controls (C) to explore the improvements in microstructures and expression of surfactant protein (SP)-A and -C at postoperative 4th, 8th, and 12th week. Result Apart from the profound metabolic changes and improvement in pulmonary function, lung volume was restored along with an improved diffusion capacity noted by thinned capillary basement membrane and decreased harmonic mean length of diffusion barrier in SG rats. The digital slices of light microscope showed the general changes brought on by the SG, including normalized basic structures, ameliorated inflammatory status, as well as reduced lipid deposition, where the hydroxyproline (HYP), triglyceride (TG) assays, and electron microscope further suggested that the improvement in alveolar structures lies in reduced collagen fibers, lipids and septal tissues, increased capillary blood, and normalized alveolar type 2 (AT2) cells. Besides, disrupted SP-A and SP-C expression were also normalized after SG. Conclusion The improvement of lung function after SG is related to the ameliorated alveolar structures, and surface protein expression induced by weight loss and improved glucose metabolism.