A mushroom-derived amino acid, ergothioneine, is a potential inhibitor of inflammation-related DNA halogenation

• Published in: Bioscience, biotechnology, and biochemistry Vol. 80; no. 2; pp. 313 - 317
• Main Authors: Asahi, Takashi, Wu, Xiaohong, Shimoda, Hiroshi, Hisaka, Shinsuke, Harada, Etsuko, Kanno, Tomomi, Nakamura, Yoshimasa, Kato, Yoji, Osawa, Toshihiko
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.02.2016
• Subjects: Agaricales - chemistry; amino acids; Animals; anti-inflammatory agent; anti-inflammatory agents; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; ascorbic acid; Ascorbic Acid - pharmacology; biotechnology; Bromates - antagonists & inhibitors; Bromates - metabolism; Coprinus comatus; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - antagonists & inhibitors; Deoxyguanosine - biosynthesis; disease progression; DNA; DNA - antagonists & inhibitors; DNA - metabolism; ergothioneine; Ergothioneine - isolation & purification; Ergothioneine - pharmacology; glutathione; Glutathione - pharmacology; Halogenation - drug effects; hypobromous acid; Hypochlorous Acid - antagonists & inhibitors; Hypochlorous Acid - metabolism; Inflammation - drug therapy; Inflammation - etiology; Inflammation - metabolism; Inflammation - pathology; Male; Mice; Mice, Hairless; mushroom; mushrooms; myeloperoxidase; Peroxidase - antagonists & inhibitors; Peroxidase - metabolism; Ultraviolet Rays - adverse effects; ergothioneine; hypobromous acid; myeloperoxidase; mushroom; anti-inflammatory agent
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1080/09168451.2015.1083396

Myeloperoxidase (MPO)-generated halogenating molecules, such as hypochlorous acid and hypobromous acid (HOBr), in inflammatory regions are postulated to contribute to disease progression. In this study, we showed that ergothioneine (EGT), derived from an edible mushroom, inhibited MPO activity as well as the formation of 8-bromo-2′-deoxyguanosine in vitro. The HOBr scavenging effect of EGT is higher than those of ascorbic acid and glutathione. We initially observed that the administration of Coprinus comatus, an edible mushroom containing a high amount of EGT, inhibited the UV-B-induced inflammatory responses and DNA halogenation, suggesting that EGT is a promising anti-inflammatory agent from mushrooms. The ergothioneine-rich Coprinus extract inhibited both MPO activity and HOBr-induced DNA damage in addition to inhibition of UV-B-induced inflammatory responses.