Effect of Amino Acids on Expression of Signal Molecules in Organotypic Culture of the Spleen

• Published in: Bulletin of experimental biology and medicine Vol. 153; no. 4; pp. 573 - 576
• Main Authors: Kontcevaya, E. A., Linkova, N. S, Chalisova, N. I., Dudkov, A. V., Sinyachkin, D. A.
• Format: Journal Article
• Language: English; Russian
• Published: Boston Springer US 01.08.2012; Springer; Springer Nature B.V
• Subjects: Amino Acids - chemistry; Amino Acids - pharmacology; Animals; Antigens, CD - metabolism; Antigens, Differentiation, Myelomonocytic - metabolism; B cells; Biomarkers - metabolism; Biomedical and Life Sciences; Biomedicine; CD4 Antigens - metabolism; CD5 Antigens - metabolism; Cell Biology; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Glutamate; Histidine; Hydrophobic and Hydrophilic Interactions - drug effects; Image Processing, Computer-Assisted; Immunohistochemistry; Internal Medicine; Laboratory Medicine; Lymphocytes - drug effects; Male; Pathology; Rats; Rats, Wistar; Signal Transduction - drug effects; Spleen - cytology; Spleen - drug effects; T cells; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); immune cells; differentiation; proliferation; spleen; amino acids
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-012-1769-6

Opposite effects of amino acids on proliferation and differentiation of immune cells in organotypic culture of the spleen were demonstrated. It was found that methionine stimulates differentiation of CD5 + lymphocyte precursors into T-helpers, cytotoxic T lymphocytes, and B cells and induces proliferation of CD68 + -macrophages. Glutamic acid and arginine induce proliferation of B cells, while histidine and leucine promote differentiation of precursors towards cytotoxic T cells. The opposite effects of amino acids on immune cells of the spleen are related to different hydrophobicity of their side chains determining the type of intermolecular interaction.