Sources of hepatic glycogen synthesis following a milk-containing breakfast meal in healthy subjects

• Published in: Metabolism, clinical and experimental Vol. 61; no. 2; pp. 250 - 254
• Main Authors: Barosa, Cristina, Silva, Claudia, Fagulha, Ana, Barros, Luísa, Caldeira, M. Madalena, Carvalheiro, Manuela, Jones, John G.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.02.2012; Elsevier
• Subjects: acetaminophen; Adult; Animals; Biological and medical sciences; body water; breakfast; Carbon Isotopes - pharmacokinetics; deuterium; Deuterium Oxide - pharmacokinetics; Eating - physiology; Endocrinology & Metabolism; Feeding. Feeding behavior; Female; Fructosephosphates - metabolism; Fundamental and applied biological sciences. Psychology; galactose; glucose; Glucose - metabolism; Glucose - pharmacokinetics; glucose 6-phosphate; Glucose-6-Phosphate - metabolism; Glucuronides - metabolism; glycogen; glycogenesis; Health; Humans; Liver Glycogen - biosynthesis; Liver Glycogen - metabolism; Male; menthol; metabolites; Milk - metabolism; Milk - physiology; nuclear magnetic resonance spectroscopy; Tissue Distribution; tracer techniques; uridine diphosphate; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Young Adult; Breakfast; Healthy subject; Digestive system; Glycogen; Liver; Meal; Endocrinology; Milk
• ISSN: 0026-0495; 1532-8600; 1532-8600
• DOI: 10.1016/j.metabol.2011.06.022

During feeding, dietary galactose is a potential source of hepatic glycogen synthesis; but its contribution has not been measured to date. In the presence of deuterated water ( 2H 2O), uridine diphosphate (UDP)–glucose derived from galactose is not enriched, whereas the remainder derived from glucose-6-phosphate (G6P) is enriched in position 2 to the same level as body water, assuming complete G6P–fructose-6-phosphate (F6P) exchange. Hence, the difference between UDP-glucose position 2 and body water enrichments reflects the contribution of galactose to glycogen synthesis relative to all other sources. In study 1, G6P-F6P exchange in 6 healthy subjects was quantified by supplementing a milk-containing breakfast meal with 10 g of [U- 2H 7]glucose and quantifying the depletion of position 2 enrichment in urinary menthol glucuronide. In study 2, another 6 subjects ingested 2H 2O and acetaminophen followed by an identical breakfast meal with 10 g of [1- 13C]glucose to resolve direct/indirect pathways and galactose contributions to glycogen synthesis. Metabolite enrichments were determined by 2H and 13C nuclear magnetic resonance. In study 1, G6P-F6P exchange approached completion; therefore, the difference between position 2 and body water enrichments in study 2 (0.20% ± 0.03% vs 0.27% ± 0.03%, P < .005) was attributed to galactose glycogenesis. Dietary galactose contributed 19% ± 3% to glycogen synthesis. Of the remainder, 58% ± 5% was derived from the direct pathway and 22% ± 4% via the indirect pathway. The contribution of galactose to hepatic glycogen synthesis was resolved from that of direct and indirect pathways using a combination of 2H 2O and [1- 13C]glucose tracers.