A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons
Current electrophysiological or optical techniques cannot reliably perform simultaneous intracellular recordings from more than a few tens of neurons. Here we report a nanoelectrode array that can simultaneously obtain intracellular recordings from thousands of connected mammalian neurons in vitro....
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Published in | Nature biomedical engineering Vol. 4; no. 2; pp. 232 - 241 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
01.02.2020
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Current electrophysiological or optical techniques cannot reliably perform simultaneous intracellular recordings from more than a few tens of neurons. Here we report a nanoelectrode array that can simultaneously obtain intracellular recordings from thousands of connected mammalian neurons in vitro. The array consists of 4,096 platinum-black electrodes with nanoscale roughness fabricated on top of a silicon chip that monolithically integrates 4,096 microscale amplifiers, configurable into pseudocurrent-clamp mode (for concurrent current injection and voltage recording) or into pseudovoltage-clamp mode (for concurrent voltage application and current recording). We used the array in pseudovoltage-clamp mode to measure the effects of drugs on ion-channel currents. In pseudocurrent-clamp mode, the array intracellularly recorded action potentials and postsynaptic potentials from thousands of neurons. In addition, we mapped over 300 excitatory and inhibitory synaptic connections from more than 1,700 neurons that were intracellularly recorded for 19 min. This high-throughput intracellular-recording technology could benefit functional connectome mapping, electrophysiological screening and other functional interrogations of neuronal networks.
An electronic interface with 4,096 electrodes can intracellularly record postsynaptic potentials and action potentials from thousands of connected mammalian neurons in vitro. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 H.P., D.H., J.A., T.Y., and K.K. conceived and designed the experiments. J.A. and L.Q. designed the CMOS IC, J.A., Y.K. and W.W. designed the interface electronics, and T.Y., S.B., and K.K. performed post-fabrication and device packaging. J.A., T.Y., K.K., and R.G. performed the experiments, and J.A., T.Y., K.K., H.P. and D.H. analyzed the data. H.P. and D.H. supervised the project. J.A., T.Y., K.K., D.H., and H.P. wrote the manuscript, and all authors read and discussed it. Author contributions |
ISSN: | 2157-846X 2157-846X |
DOI: | 10.1038/s41551-019-0455-7 |