Susceptibilities against bovine lactoferrin with microorganisms isolated from mastitic milk

• Published in: Journal of Veterinary Medical Science Vol. 66; no. 10; pp. 1267 - 1269
• Main Authors: Lee, N.Y. (Hokkaido Univ., Sapporo (Japan)), Kawai, K, Nakamura, I, Tanaka, T, Kumura, H, Shimazaki, K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.10.2004; Japan Science and Technology Agency
• Subjects: Animals; antimicrobial activity; ANTIMICROBIAL PROPERTIES; Bacteria - drug effects; CATTLE; Cattle - metabolism; Escherichia coli; Female; Fungi - drug effects; Japan; Klebsiella pneumoniae; LACTOFERRIN; Lactoferrin - metabolism; Lactoferrin - pharmacology; mastitic milk; MASTITIS; Mastitis, Bovine - microbiology; Microbial Sensitivity Tests; MICROORGANISMS; MILK; Milk - microbiology; Prototheca zopfii; Staphylococcus aureus; Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.66.1267

Antibacterial effects of bovine lactoferrin were studied in vitro against microorganisms isolated from mastitis milk in Tokachi area, Hokkaido, Japan. Microorganisms isolated were Eschelichia colt (11 isolates), Klebsiella pneumoniae (5 isolates), enterococci (8 isolates), Staphylococcus aureus (10 isolates), coagulase negative staphylococci (CNS, 13 isolates), streptococci (11 isolates), Prorotheca zopfli (7 isolates) and yeast-like fungi (9 isolates). Lactoferrin has been known as a multifunctional protein and its antimicrobial effect is one of the most essential function of it. In order to compare their susceptibilities against lactoferrin, the minimal inhibitory concentration values were estimated by a microplate assay method using 96-well microplate, which involved measuring the optical density of the cultures. Protatheca zopfli was highly sensitive to bovine lactoferrin and complete inhibition of this microorganism was observed even at the low concentration of 7 micro g/ml. On the other hand, E. colt and enterococci showed resistance against lactoferrin action and staphylococci showed strain-dependent resistance.