Determination of free and conjugated bile acids in serum of Apoe(-/-) mice fed different lingonberry fractions by UHPLC-MS

• Published in: Scientific reports Vol. 9; no. 1; p. 3800
• Main Authors: Ghaffarzadegan, Tannaz, Essén, Sofia, Verbrugghe, Phebe, Marungruang, Nittaya, Hållenius, Frida Fåk, Nyman, Margareta, Sandahl, Margareta
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 07.03.2019; Nature Publishing Group UK
• Subjects: Acids; Agricultural Science, Forestry and Fisheries; Agricultural Sciences; Apolipoprotein E; Bile; Bile acid; Bile acids; Biologi; Biological Sciences; Cellulose; Chenodeoxycholic acid; Cholesterol; Cholic acid; Deoxycholic acid; Diet; Food Science; Glycine; High fat diet; High-performance liquid chromatography; Intestinal microflora; Lantbruksvetenskap och veterinärmedicin; Lantbruksvetenskap, skogsbruk och fiske; Lingonberry; Lipid metabolism; Livsmedelsvetenskap; Low fat diet; Mass spectroscopy; Metabolism; Mice; Microbiota; Natural Sciences; Naturvetenskap; Nutrient deficiency; Taurine; UHPLC-MS; Ursodeoxycholic acid; Vaccinium vitis-idaea
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-40272-8

Bile acids (BAs) are known to be involved in cholesterol metabolism but interactions between the diet, BA profiles, gut microbiota and lipid metabolism have not been extensively explored. In the present study, primary and secondary BAs including their glycine and taurine-conjugated forms were quantified in serum of Apoe-/- mice by protein precipitation followed by reversed phase ultra-high-performance liquid chromatography and QTOF mass spectrometry. The mice were fed different lingonberry fractions (whole, insoluble and soluble) in a high-fat setting or cellulose in a high and low-fat setting. Serum concentrations of BAs in mice fed cellulose were higher with the high-fat diet compared to the low-fat diet (20-70%). Among the lingonberry diets, the diet containing whole lingonberries had the highest concentration of chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA), tauro-ursodeoxycholic acid (T-UDCA), α and ω-muricholic acids (MCA) and tauro-α-MCA (T-α-MCA), and the lowest concentration of tauro-cholic acid (T-CA), deoxycholic acid (DCA) and tauro-deoxycholic acid (T-DCA). The glycine-conjugated BAs were very similar with all diets. CDCA, UDCA and α-MCA correlated positively with Bifidobacterium and Prevotella, and T-UDCA, T-α-MCA and ω-MCA with Bacteroides and Parabacteroides.