Structure of flocs of latex particles formed by addition of protein from Moringa seeds

• Published in: Colloids and surfaces. A, Physicochemical and engineering aspects Vol. 460; pp. 460 - 467
• Main Authors: Hellsing, Maja S., Kwaambwa, Habauka M., Nermark, Fiona M., Nkoane, Bonang B.M., Jackson, Andrew J., Wasbrough, Matthew J., Berts, Ida, Porcar, Lionel, Rennie, Adrian R.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 20.10.2014
• Subjects: Flocculation; Fractal aggregates; Fysik; Natural Sciences; Naturvetenskap; Physical Sciences; Protein adsorption; Fractal aggregates; Protein adsorption; Flocculation
• ISSN: 0927-7757; 1873-4359; 1873-4359
• DOI: 10.1016/j.colsurfa.2013.11.038

•Moringa oleifera seed protein binds strongly to polystyrene latex.•Seed protein is an effective flocculent creating very dense flocs.•Fractal dimensions of flocs increase with particle concentration.•Floc densities are higher than those found with simple ionic coagulants. Proteins extracted from the seeds of Moringa trees are effective flocculents for particles dispersed in water and are attractive as a natural and sustainable product for use in water purification. Studies with a model system consisting of polystyrene latex particles have shown that the protein adsorbs to the surface and causes flocculation as unusually dense aggregates. Small-angle neutron scattering that exploits contrast matching of deuterated latex particles dispersed in D2O to highlight bound protein has shown that the adsorbed amount reaches about 3mgm−2. The particles form very compact flocs that are characterized by fractal dimensions that approach the theoretical maximum of 3. Ultra small-angle neutron scattering allows these flocs to be characterized for a range of particle and protein concentrations. Proteins from two species of Moringa trees were investigated. The protein from Moringa stenopetala seeds gave rise to slightly lower fractal dimensions compared to Moringa oleifera, but still much larger than values observed for conventional ionic or polymeric flocculents that are in the range 1.75–2.3. Compact flocs are desirable for efficient separation of impurities and dewatering of sludge as well as other applications. A trend of increasing fractal dimension with particle concentration was observed when M. stenopetala seed protein was used and this resembles the behaviour predicted in Brownian dynamics simulation of flocculation.