Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes

• Published in: Planta Vol. 245; no. 4; pp. 749 - 764
• Main Authors: Peters, Leila P., Carvalho, Giselle, Vilhena, Milca B., Creste, Silvana, Azevedo, Ricardo A., Monteiro-Vitorello, Claudia B.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Science + Business Media 01.04.2017; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Agriculture; Biomedical and Life Sciences; Developmental stages; Disease Susceptibility - metabolism; Ecology; Enzymatic activity; Forestry; Gene Expression Regulation, Plant - physiology; Genotype; Genotypes; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Life Sciences; Lipid Peroxidation; ORIGINAL ARTICLE; Pathogens; Peroxidation; Plant Diseases - microbiology; Plant Sciences; Reactive Oxygen Species - metabolism; Respiratory Burst - physiology; Saccharum - microbiology; Saccharum - physiology; Sugarcane; Ustilago - pathogenicity; Biotic stress; Reactive oxygen species; Phytopathogen; Hydrogen peroxide; Antioxidant enzymes; Sporisorium scitamineum
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-016-2642-z

Sporisorium scitamineum is the causal agent of sugarcane smut disease. In this study, we characterized sugarcane reactive oxygen species (ROS) metabolism in response to the pathogen in smut-resistant and-susceptible genotypes. Sporisorium scitamineum teliospore germination and appressorium formation coincided with H₂O₂ accumulation in resistant plants. The superoxide dismutase (SOD) activity was not responsive in any of the genotypes; however, a higher number of isoenzymes were detected in resistant plants. In addition, related to resistance were lipid peroxidation, a decrease in catalase (CAT), and an increase in glutathione S-transferase (GST) activities and an earlier transcript accumulation ofROS marker genes (CAT3, CATA, CATB, GST31, GSTt3, and peroxidase 5-like). Furthermore, based on proteomic data, we suggested that the source of the increased hydrogen peroxide (H₂O₂) may be due to a protein of the class III peroxidase, which was inhibited in the susceptible genotype. H₂O₂ is sensed and probably transduced through overlapping systems related to ascorbate–glutathione and thioredoxin to influence signalling pathways, as revealed by the presence of thioredoxin h-type, ascorbate peroxidase, and guanine nucleotide-binding proteins in the infected resistant plants. Altogether, our data depicted the balance of the oxidative burst and antioxidant enzyme activity in the outcome of this interaction.