Identification of boric acid as a novel chemoattractant and elucidation of its chemoreceptor in Ralstonia pseudosolanacearum Ps29
Chemotaxis enables bacteria to move toward more favorable environmental conditions. We observed chemotaxis toward boric acid by Ralstonia pseudosolanacearum Ps29. At higher concentrations, the chemotactic response of R. pseudosolanacearum toward boric acid was comparable to or higher than that towar...
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Published in | Scientific reports Vol. 7; no. 1; pp. 8609 - 10 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
17.08.2017
Nature Publishing Group Nature Portfolio |
Subjects | |
Online Access | Get full text |
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Summary: | Chemotaxis enables bacteria to move toward more favorable environmental conditions. We observed chemotaxis toward boric acid by
Ralstonia pseudosolanacearum
Ps29. At higher concentrations, the chemotactic response of
R. pseudosolanacearum
toward boric acid was comparable to or higher than that toward L-malate, indicating that boric acid is a strong attractant for
R. pseudosolanacearum
. Chemotaxis assays under different pH conditions suggested that
R. pseudosolanacearum
recognizes B(OH)
3
(or B(OH
3
) + B(OH)
4
−
) but not B(OH)
4
−
alone. Our previous study revealed that
R. pseudosolanacearum
Ps29 harbors homologs of all 22
R. pseudosolanacearum
GMI1000
mcp
genes. Screening of 22
mcp
single-deletion mutants identified the RS_RS17100 homolog as the boric acid chemoreceptor, which was designated McpB. The McpB ligand-binding domain (LBD) was purified in order to characterize its binding to boric acid. Using isothermal titration calorimetry, we demonstrated that boric acid binds directly to the McpB LBD with a
K
D
(dissociation constant) of 5.4 µM. Analytical ultracentrifugation studies revealed that the McpB LBD is present as a dimer that recognizes one boric acid molecule. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-017-09176-3 |