The presence of high level soluble herpes virus entry mediator in sera of gastric cancer patients

• Published in: Experimental & molecular medicine Vol. 44; no. 2; pp. 149 - 158
• Main Authors: Heo, Sook-Kyoung, Ju, Seong-A, Kim, Gyu Yeol, Park, Sang-Min, Back, Sung Hun, Park, Neung-Hwa, Min, Young Joo, An, Won G, Nguyen, Thu-Ha Thi, Kim, Sun-Min, Kim, Byung-Sam
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.02.2012; Springer Nature B.V; Korean Society for Biochemistry and Molecular Biology; 생화학분자생물학회
• Subjects: Aged; Biomedical and Life Sciences; Biomedicine; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Helicobacter pylori; Herpesvirus; Humans; Male; Medical Biochemistry; Middle Aged; Molecular Medicine; Monocytes - metabolism; Neutrophils - metabolism; Original; Reactive Oxygen Species - metabolism; Receptors, Tumor Necrosis Factor, Member 14 - blood; Staphylococcus aureus; Stem Cells; Stomach Neoplasms - blood; Stomach Neoplasms - metabolism; Tumor Necrosis Factor Ligand Superfamily Member 14 - blood; 생화학; monocytes; receptors, tumor necrosis factor, member 14; cytokines; stomach neoplasms; inflammation; TNFSF14 protein, human
• ISSN: 1226-3613; 2092-6413
• DOI: 10.3858/emm.2012.44.2.010

The development of gastric cancer (GC) is closely related to chronic inflammation caused by Helicobacter pylori infection, and herpes virus entry mediator (HVEM) is a receptor expressed on the surface of leukocytes that mediates potent inflammatory responses in animal models. However, the role of HVEM in human GC has not been studied. Previously, we showed that the interaction of HVEM on human leukocytes with its ligand LIGHT induces intracellular calcium mobilization, which results in inflammatory responses including induction of proinflammatory cytokine production and anti-bacterial activities. In this study, we report that leukocytes from GC patients express lower levels of membrane HVEM (mHVEM) and have lower LIGHT-induced bactericidal activities than those from healthy controls (HC). In contrast, levels of soluble HVEM (sHVEM) in the sera of GC patients were significantly higher than in those of HC. We found that monocyte membrane-bound HVEM is released into the medium when cells are activated by proinflammatory cytokines such as TNF-α and IL-8, which are elevated in the sera of GC patients. mHVEM level dropped in parallel with the release of sHVEM, and release was completely blocked by the metalloprotease inhibitor, GM6001. We also found that the low level of mHVEM on GC patient leukocytes was correlated with low LIGHT-induced bactericidal activities against H. pylori and S. aureus and production of reactive oxygen species. Our results indicate that mHVEM on leukocytes and sHVEM in sera may contribute to the development and/or progression of GC.