Anti- Helicobacter pylori metabolites from Rhizoctonia sp. Cy064, an endophytic fungus in Cynodon dactylon

• Published in: Fitoterapia Vol. 75; no. 5; pp. 451 - 456
• Main Authors: Ma, Y.M, Li, Y, Liu, J.Y, Song, Y.C, Tan, R.X
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2004; Elsevier
• Subjects: Anti- Helicobacter pylori; Anti-Infective Agents - administration & dosage; Anti-Infective Agents - pharmacology; Anti-Infective Agents - therapeutic use; Biological and medical sciences; Cynodon - microbiology; Cynodon dactylon; Endophyte; General pharmacology; Helicobacter pylori; Helicobacter pylori - classification; Helicobacter pylori - drug effects; Humans; Medical sciences; Microbial Sensitivity Tests; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytotherapy; Plant Leaves - microbiology; Rhizoctonia; Rhizoctonia sp; Rhizoctonic acid; Endophyte; Cynodon dactylon; Rhizoctonic acid; Rhizoctonia sp; Anti- Helicobacter pylori; Monocotyledones; Rhizoctonia; Spirillales; Anti-Helicobacter pylori; Metabolite; Pharmacognosy; Spirillaceae; Medicinal plant; Fungi; Helicobacter pylori; Gramineae; Angiospermae; Bacteria; Spermatophyta; Fungi Imperfecti; Thallophyta
• ISSN: 0367-326X
• DOI: 10.1016/j.fitote.2004.03.007

A new benzophenone, named rhizoctonic acid ( 1), together with three known compounds monomethylsulochrin ( 2), ergosterol ( 3) and 3β,5α,6β-trihydroxyergosta-7,22-diene ( 4) were isolated through bioassay-guided fractionations from the culture of Rhizoctonia sp. (Cy064), an endophytic fungus in the leaf of Cynodon dactylon. The structure of the new acid 1 was elucidated to be 5-hydroxy-2-(2-hydroxy-6-methoxy-4-methylbenzoyl)-3-methoxybenzoic acid by a combination of spectral analyses. Furthermore, the structure of monomethylsulochrin 2 was confirmed by 13C-NMR analysis. All four metabolites were subjected to a more detailed in vitro assessment of their antibacterial action against five clinically isolated and one reference (ATCC 43504) Helicobacter pylori strains.