PGPR strain Paenibacillus polymyxa SQR-21 potentially benefits watermelon growth by re-shaping root protein expression

• Published in: AMB Express Vol. 7; no. 1; p. 104
• Main Authors: E, Yaoyao, Yuan, Jun, Yang, Fang, Wang, Lei, Ma, Jinghua, Li, Jing, Pu, Xiaowei, Raza, Waseem, Huang, Qiwei, Shen, Qirong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 25.05.2017; Springer Nature B.V; SpringerOpen
• Subjects: ADP-ribosylation factor; Ascorbic acid; Bacteria; Biological control; Biomedical and Life Sciences; Biotechnology; Carbohydrates; Citrullus; Disease resistance; Fusarium; Glucose 6 phosphate dehydrogenase; Glucose transport; Glucosephosphate dehydrogenase; Glutathione; Glutathione transferase; Hydroponic split-roots; Inoculation; L-Ascorbate peroxidase; Life Sciences; Metabolism; Microbial Genetics and Genomics; Microbiology; Original; Original Article; Oxidation; Paenibacillus polymyxa; Peroxidase; Phospholipase; Phospholipase D; Photosynthesis; Proteins; Proteomics; Signal transduction; Thioredoxin; Thioredoxin peroxidase; Ubiquitin; Wilt; Biological control; Proteomics; Hydroponic split-roots; Paenibacillus polymyxa
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-017-0403-4

Paenibacillus polymyxa (SQR-21) is not only a plant growth-promoting rhizobacteria, but also an effective biocontrol agent against Fusarium wilt disease of watermelon. For the better understanding and clarifying the potential mechanisms of SQR-21 to improve watermelon growth and disease resistance, a split-root methodology in hydroponic and LC–MS technology with the label free method was used to analyze the key root proteins involved in watermelon metabolism and disease resistance after the inoculation of SQR-21. Out of 623 identified proteins, 119 proteins were differentially expressed when treatment (SQR-21 inoculation) and control (no bacterial inoculation) were compared. Among those, 57 and 62 proteins were up-regulated and down-regulated, respectively. These differentially expressed proteins were identified to be involved in signal transduction (ADP-ribosylation factor, phospholipase D), transport (aspartate amino-transferase), carbohydratemetabolic (glucose-6-phosphate dehydrogenase, UDP-glucose pyrophosphorylase), defense and response to stress (glutathione S-transferase, Ubiquitin-activating enzyme E1), and oxidation–reduction process (thioredoxin peroxidase, ascorbate peroxidase). The results of this study indicated that SQR-21 inoculation on the watermelon roots benefits plant by inducing the expression of several proteins involved in growth, photosynthesis, and other metabolic and physiological activities.