Streptomyces sanglieri which colonised and enhanced the growth of Elaeis guineensis Jacq. seedlings was antagonistic to Ganoderma boninense in in vitro studies

• Published in: Journal of industrial microbiology & biotechnology Vol. 43; no. 4; pp. 485 - 493
• Main Authors: Nur Azura, A. B, Yusoff, M, Tan, G. Y. A, Jegadeesh, R, Appleton, D. R, Vikineswary, S
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2016; Springer Nature B.V
• Subjects: agar; Analysis; antibiotics; Antifungal Agents; antifungal properties; Arecaceae - growth & development; Arecaceae - microbiology; Bacteria; Biochemistry; Bioinformatics; Biomedical and Life Sciences; Biotechnology; casein; Chromatography, High Pressure Liquid; cycloheximide; Disease; Elaeis guineensis; Environmental Microbiology; ethyl acetate; Flowers & plants; Fungi; Ganoderma; Ganoderma - physiology; Ganoderma boninense; Genetic Engineering; Genetic testing; greenhouse experimentation; high performance liquid chromatography; in vitro studies; Infections; Inorganic Chemistry; Laboratories; Leaves; Life Sciences; Methods; Microbiology; Palm Oil; Pest Control, Biological - methods; Phylogenetics; Plant diseases; Plant Diseases - microbiology; Plant Oils; Plant Roots - growth & development; Plant Roots - microbiology; roots; scanning electron microscopy; seedling growth; seedlings; Seedlings - growth & development; Seedlings - microbiology; Software; spores; starch; stem rot; Streptomyces; Streptomyces - growth & development; Streptomyces - physiology; Studies; Surgery; Vegetable oils; Cycloheximide; Antagonistic effect; Actiphenol; Streptomyces sanglieri; Ganoderma boninense
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-015-1724-4

Actinomycete strain AUM 00500 was 99.5 % similar to Streptomyces sanglieri NBRC 100784ᵀ and was evaluated for antagonistic activity towards Ganoderma boninense, the causative fungus of basal stem rot of oil palm. The strain showed strong antifungal activity towards G. boninense in in vitro and SEM analysis showed various modes of inhibition of the fungus. Ethyl acetate extracts of single culture and inhibition zone of cross-plug culture by HPLC indicated that strain AUM 00500 produced two different antibiotics of the glutarimide group namely cycloheximide and actiphenol. In greenhouse trials, oil palm seed treated with spores of S. sanglieri strain AUM 00500 at 10⁹ cfu/ml showed significant (P < 0.05) increase in oil palm seedlings growth when compared to the control. Streptomyces sanglieri strain AUM 00500 successfully colonised the epidermal surface of the roots of treated oil palm seedlings and it was recovered from root fragments plated on starch casein agar.