Study on a luminol-based electrochemiluminescent sensor for label-free DNA sensing

Automatic, inexpensive, simple and sensitive methods for DNA sensing and quantification are highly desirable for biomedical research. The rapid development of both the fundamentals and applications of electrochemiluminescence (ECL) over the past years has demonstrated its potential for analytical an...

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Bibliographic Details
Published inSensors (Basel, Switzerland) Vol. 10; no. 10; pp. 9481 - 9492
Main Authors Chu, Hai-Hong, Yan, Ji-Lin, Tu, Yi-Feng
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.10.2010
Molecular Diversity Preservation International (MDPI)
Subjects
Analytical chemistry
Aqueous solutions
Carbon
Deoxyribonucleic acid
DNA
DNA - analysis
electrochemiluminescence
Electrochemistry - instrumentation
Electrodes
Electrons
Genetic testing
Gold - chemistry
Hybridization
label-free sensing
Luminescent Measurements - instrumentation
Luminol - chemistry
Metal Nanoparticles - chemistry
nano-prefunctional sensor
Nanomaterials
Nanoparticles
Nanotubes, Carbon - chemistry
quenching effect
Sensors
China
quenching effect
electrochemiluminescence
label-free sensing
nano-prefunctional sensor
DNA
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Summary:Automatic, inexpensive, simple and sensitive methods for DNA sensing and quantification are highly desirable for biomedical research. The rapid development of both the fundamentals and applications of electrochemiluminescence (ECL) over the past years has demonstrated its potential for analytical and bio-analytical chemistry. This paper reports the quenching effect of DNA on the ECL of luminol and the further development of a DNA sensing device. With the pre-functionalization by a composite of carbon nano-tubes (CNTs) and Au nanoparticles (AuNPs), the sensor provides a novel and valuable label-free approach for DNA sensing. Here the ECL intensity was remarkably decreased when more than 1.0 × 10(-12) molar of DNA were adsorbed on the sensor. Linearity of the DNA amount with the reciprocal of ECL intensity was observed. A saturated sensor caused a 92.8% quenching effect. The research also proposes the mechanism for the quenching effect which could be attributed to the interaction between luminol and DNA and the elimination of reactive oxygen species (ROSs) by DNA.
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ISSN:1424-8220
1424-8220
DOI:10.3390/s101009481