Using Omniscan-Loaded Nanoparticles as a Tumor-Targeted MRI Contrast Agent in Oral Squamous Cell Carcinoma by Gelatinase-Stimuli Strategy

• Published in: Nanoscale research letters Vol. 14; no. 1; pp. 395 - 9
• Main Authors: Gao, Antian, Teng, Yuehui, Seyiti, Pakezhati, Yen, Yingtzu, Qian, Hanqing, Xie, Chen, Li, Rutian, Lin, Zitong
• Format: Journal Article
• Language: English
• Published: New York Springer US 30.12.2019; Springer Nature B.V; SpringerOpen
• Subjects: Bile; Cell culture; Chemistry and Materials Science; Collagen; Contrast agents; Gelatinase; Gelatinase-stimuli; Magnetic resonance imaging; Materials Science; Molecular Medicine; Morphology; Nano Express; Nanochemistry; Nanoparticles; Nanoscale Science and Technology; Nanotechnology; Nanotechnology and Microengineering; Omniscan; Oral squamous cell carcinoma; Squamous cell carcinoma; Stimuli; Stress concentration; Tumor-targeted MRI contrast agent; Tumors; Nanoparticles; Omniscan; Gelatinase-stimuli; Oral squamous cell carcinoma; Tumor-targeted MRI contrast agent
• ISSN: 1931-7573; 1556-276X
• DOI: 10.1186/s11671-019-3214-5

In this study, the tumor-targeted MRI contrast agent was prepared with gelatinase-stimuli nanoparticles (NPs) and Omniscan (Omn) by double emulsion method. The size, distribution, morphology, stability, drug loading, and encapsulation efficiency of Omn-NPs were characterized. The macroscopic and microscopic morphological changes of NPs in response to gelatinases (collagenases IV) were observed. The MR imaging using Omn-NPs as a contrast agent was evaluated in the oral squamous cell carcinoma models with Omn as a control. We found clear evidence that the Omn-NPs were transformed by gelatinases and the signal of T1-weighted MRI sequence showed that the tumor-to-background ratio was significantly higher in Omn-NPs than in Omn. The peak point of time after injection was much later for Omn-NPs than Omn. This study demonstrates that Omn-NPs hold great promise as MRI contrast agent with improved specificity and prolonged circulation time based on a relatively simple and universal strategy.