Reconstructing Mandibular Defects Using Autologous Tissue-Engineered Tooth and Bone Constructs

• Published in: Journal of oral and maxillofacial surgery Vol. 67; no. 2; pp. 335 - 347
• Main Authors: Abukawa, Harutsugi, DDS, PhD, Zhang, Weibo, DMD, PhD, Young, Conan S., PhD, Asrican, Rose, MS, Vacanti, Joseph P., MD, Kaban, Leonard B., DMD, MD, Troulis, Maria J., DDS, MSc, Yelick, Pamela C., PhD
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.02.2009; Elsevier
• Subjects: Amelogenin - biosynthesis; Animals; Biological and medical sciences; Bone Regeneration - physiology; Cell Culture Techniques; Cell Differentiation; Cells, Cultured; Dentistry; Epithelial Cells - transplantation; Feasibility Studies; Male; Mandible - cytology; Mandible - surgery; Medical sciences; Mesenchymal Stem Cells - cytology; Models, Animal; Odontogenesis - physiology; Osteoblasts - transplantation; Otorhinolaryngology. Stomatology; Phosphoproteins - biosynthesis; Pilot Projects; Regeneration - physiology; Surgery; Swine; Swine, Miniature; Tissue Engineering - methods; Tissue Scaffolds; Tooth - cytology; Tooth - metabolism; Tooth Germ - transplantation; Tissue; Mandible; Treatment; Stomatology; Surgery; Diseases of the osteoarticular system; Teeth; Bone defect; Autologous system; Tooth; Bone
• ISSN: 0278-2391; 1531-5053
• DOI: 10.1016/j.joms.2008.09.002

Purpose Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model. Materials and Methods Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses. Results Small tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues. Conclusions This pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.