Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice
Background Abscisic acid (ABA) plays crucial roles in regulating plant growth and development, especially in responding to abiotic stress. The pyrabactin resistance-like (PYL) abscisic acid receptor family has been identified and widely characterized in Arabidopsis . However, PYL families in rice we...
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Published in | Rice (New York, N.Y.) Vol. 8; no. 1; p. 28 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
11.09.2015
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Background
Abscisic acid (ABA) plays crucial roles in regulating plant growth and development, especially in responding to abiotic stress. The pyrabactin resistance-like (PYL) abscisic acid receptor family has been identified and widely characterized in
Arabidopsis
. However, PYL families in rice were largely unknown. In the present study, 10 out of 13 PYL orthologs in rice (
OsPYL
) were isolated and investigated.
Results
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that expression of
OsPYL
genes is tissue-specific and display differential response to ABA treatment, implying their functional diversity. The interaction between 10 OsPYL members and 5 protein phosphatase 2C in rice (OsPP2C) members was investigated in yeast two-hybrid and tobacco transient expression assays, and an overall interaction map was generated, which was suggestive of the diversity and complexity of ABA-sensing signaling in rice. To study the biological function of
OsPYLs
, two
OsPYL
genes (
OsPYL3
and
OsPYL9
) were overexpressed in rice. Phenotypic analysis of
OsPYL3
and
OsPYL9
transgenic rice showed that
OsPYLs
positively regulated the ABA response during the seed germination. More importantly, the overexpression of
OsPYL3
and
OsPYL9
substantially improved drought and cold stress tolerance in rice.
Conclusion
Taken together, we comprehensively uncovered the properties of
OsPYLs
, which may be good candidates for the improvement of abiotic stress tolerance in rice. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1939-8425 1939-8433 1934-8037 |
DOI: | 10.1186/s12284-015-0061-6 |