Natural Membrane Differentiates Human Adipose-Derived Mesenchymal Stem Cells to Neurospheres by Mechanotransduction Related to YAP and AMOT Proteins

• Published in: Membranes (Basel) Vol. 11; no. 9; p. 687
• Main Authors: de Oliveira, Nathalia Barth, Irioda, Ana Carolina, Stricker, Priscila Elias Ferreira, Mogharbel, Bassam Felipe, da Rosa, Nádia Nascimento, Dziedzic, Dilcele Silva Moreira, de Carvalho, Katherine Athayde Teixeira
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 05.09.2021; MDPI
• Subjects: Adipose tissue; adipose-derived mesenchymal stem cells; angiomotin; Biomarkers; Biopolymers; Cell differentiation; Cell lines; Cytoplasm; Differentiation; Epigenetics; Flasks; Flow cytometry; Glial fibrillary acidic protein; Growth factors; Homeostasis; Immunocytochemistry; Mechanotransduction; membrane; Membranes; Mesenchymal stem cells; Nestin; neural precursor; Neural stem cells; Neurodegenerative diseases; Neurospheres; polyisoprene; Polymerase chain reaction; Polystyrene; Polystyrene resins; Proteins; Regenerative medicine; Stem cells; Surface markers; Tissue engineering; Tubulin; YAP; Yes-associated protein; St Louis Missouri; United States > US
• ISSN: 2077-0375; 2077-0375
• DOI: 10.3390/membranes11090687

Adipose tissue-derived mesenchymal stem cells (ADMSCs) are promising candidates for regenerative medicine, as they have good cell yield and can differentiate into several cell lines. When induced to the neuronal differentiation, they form neurospheres composed of neural precursors (NPs) that can be an alternative in treating neurodegenerative diseases. This study aimed to characterize NPs from neurospheres obtained after seeding ADMSCs on a natural polyisoprene-based membrane. The ADMSCs were isolated from adipose tissue by enzymatic dissociation, were subjected to trilineage differentiation, and were characterized by flow cytometry for specific ADMSC surface markers. For neuronal differentiation, the cells were seeded on polystyrene flasks coated with the membrane and were characterized by immunocytochemistry and RT-PCR. The results demonstrated that the isolated cells showed characteristics of ADMSCs. At 15 to 25 days, ADMSCs seeded on the natural membrane developed neurospheres. Then, after dissociation, the cells demonstrated characteristic neuronal markers expressed on NPs: nestin, ß-III tubulin, GFAP, NeuN, and the YAP1/AMOT in the cytoplasm. In conclusion, it was demonstrated that this membrane differentiates the ADMSCs to NPs without any induction factors, and suggests that their differentiation mechanisms are related to mechanotransduction regulated by the YAP and AMOT proteins.