GABAergic Interneurons are Required for Generation of Slow CA1 Oscillation in Rat Hippocampus

• Published in: Neuroscience bulletin Vol. 32; no. 4; pp. 363 - 373
• Main Authors: Xu, Yuan, Wang, Lidan, Liu, Yu-zhang, Yang, Yan, Xue, Xiaolin, Wang, Zhiru
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.08.2016; Springer
• Subjects: Action Potentials - drug effects; Action Potentials - physiology; Anatomy; Anesthesiology; Animals; Animals, Newborn; Bicuculline - pharmacology; Biological Clocks - drug effects; Biological Clocks - physiology; Biomedical and Life Sciences; Biomedicine; Brain; CA1 Region, Hippocampal - cytology; CA1 Region, Hippocampal - drug effects; CA1 Region, Hippocampal - physiology; Dose-Response Relationship, Drug; Electric Stimulation; Excitatory Amino Acid Antagonists - pharmacology; GABA; GABA Modulators - pharmacology; GABA-A Receptor Antagonists - pharmacology; GABAA受体; GABAergic Neurons - drug effects; GABAergic Neurons - physiology; Human Physiology; In Vitro Techniques; Interneurons - drug effects; Interneurons - physiology; Midazolam - pharmacology; Morpholines - pharmacology; Neurology; Neurosciences; Original; Original Article; Pain Medicine; Patch-Clamp Techniques; Quinoxalines - pharmacology; Rats; Rats, Sprague-Dawley; 全细胞记录; 受体介导; 大鼠; 振荡; 海马CA1区; 神经元; 荷包牡丹碱; GABAergic interneuron; GABA; receptor; Synchronization; Hippocampus; Oscillation; GABAA receptor
• ISSN: 1673-7067; 1995-8218
• DOI: 10.1007/s12264-016-0049-2

Neuronal oscillations are fundamental to hip- pocampal function. It has been shown that GABAergic interneurons make an important contribution to hippocampal oscillations, but the underlying mechanism is not well understood. Here, using whole-cell recording in the complete hippocampal formation isolated from rats at postnatal days 14-18, we showed that GABAA receptormediated activity enhanced the generation of slow CA1 oscillations. In vitro, slow oscillations （0.5-1.5 Hz） were generated in CA1 neurons, and they consisted primarily of excitatory rather than inhibitory membrane-potential changes. These oscillations were greatly reduced by blocking GABAA receptor-mediated activity with bicuculline and were enhanced by increasing such activity with midazolam, suggesting that interneurons are required for oscillation generation. Consistently, CA1 fast-spiking interneurons were found to generate action potentials usually preceding those in CA1 pyramidal cells. These findings indicate a GABAA receptor-based mechanism for the generation of the slow CA1 oscillation in the hippocampus.