Apoptosis of phagocytic cells induced by Candida albicans and production of IL-10

• Published in: FEMS immunology and medical microbiology Vol. 42; no. 2; pp. 219 - 224
• Main Authors: Gasparoto, Thaı́s Helena, Gaziri, Luis Carlos Jabur, Burger, Eva, de Almeida, Ricardo Sérgio Couto, Felipe, Ionice
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.10.2004; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Animals; Apoptosis; Biological and medical sciences; Candida albicans; Candida albicans - pathogenicity; Candidiasis - immunology; Candidiasis - microbiology; Cell death; Exudation; Fundamental and applied biological sciences. Psychology; IL-10; Inoculation; Inoculum; Interleukin 10; Interleukin-10 - biosynthesis; Leukocytes (neutrophilic); Macrophages; Macrophages, Peritoneal - immunology; Macrophages, Peritoneal - microbiology; Macrophages, Peritoneal - physiology; Male; Mice; Microbiology; Miscellaneous; Mycology; Necrosis; Peritoneum; Phagocytes; Candida albicans; IL-10; Apoptosis; Necrosis; Fungi; Yeast; Immunology; Microbiology; Cell death; Cytokine; Interleukin 10; Fungi Imperfecti; Thallophyta
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1016/j.femsim.2004.05.006

Macrophages co-incubated with Candida albicans strain CR1 in vitro showed early signs of apoptosis, but evolved to necrosis after 2 h. In this study, we investigated whether strain CR1 caused apoptosis or necrosis of macrophages after its inoculation into mice peritoneal cavity, and whether this correlated with the secretion of IL-10. Peritoneal macrophages from mice that received an inoculum of C. albicans CR1 showed signs of apoptosis and necrosis from 30 min to 2 h afterwards, whereas heat-killed C. albicans did not cause those effects. IL-10 production was low during the first 6 h post-infection, when macrophages predominated in the peritoneal exudate, whereas its higher production after 24 h correlated with an increase of neutrophils in the exudate. Treatment of CR1 with pepstatin (an inhibitor of proteinases) prevented the process of apoptosis and significantly reduced IL-10 production, suggesting that the increased production of IL-10 was caused by processes occurring during the initial phase of infection, such as apoptosis, necrosis and uptake of death cells.