Detection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03

• Published in: FEMS microbiology letters Vol. 223; no. 1; pp. 47 - 51
• Main Authors: Cho, Soo-Jin, Lee, Sam Keun, Cha, Byeong Jin, Kim, Young Hwan, Shin, Kwang-Soo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 06.06.2003; Blackwell Publishing Ltd; Blackwell
• Subjects: agarose; Anthracnose; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - pharmacology; antibiotics; antifungal agents; Antifungal Agents - analysis; Antifungal Agents - pharmacology; Bacillus subtilis; Bacillus subtilis - chemistry; Bacillus subtilis - genetics; Biological and medical sciences; biological control agents; butanol; culture media; desorption; Fundamental and applied biological sciences. Psychology; fungi; Gloeosporium; Gloeosporium gloeosporioides; ionization; iturin; Iturin A; lipopeptides; mass spectrometry; Microbiology; Miscellaneous; Mitosporic Fungi - drug effects; Mitosporic Fungi - growth & development; molecular weight; Mycology; Peptides; Peptides, Cyclic; RNA, Ribosomal, 16S - genetics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; thin layer chromatography; Anthracnose; Iturin A; Bacillus subtilis; Gloeosporium gloeosporioides; Bacillaceae; Strain; Fungi; Bacillales; Bacteria; Gloeosporium; Fungi Imperfecti; Detection; Thallophyta
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(03)00329-X

The Bacillus subtilis strain KS03 was isolated, and identified as a biological control agent that inhibits the anthracnose disease fungus Gloeosporium gloeosporioides. The antifungal compound was purified from its culture broth through butanol extraction, diethylaminoethyl (DEAE) Sepharose CL-6B chromatography, and preparative thin layer chromatography. Tandem mass spectrometric analyses (MS/MS), with matrix-assisted laser desorption ionization (MALDI) time-of-fight/time-of-flight (TOF/TOF) mass spectrometry, showed that the antifungal compound was iturin A, a cyclic lipopeptide antibiotic. The major compound, with a molecular mass of 1042 Da, was identified as iturin A 2.