Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: an evaluation of four procedures

• Published in: Clinical microbiology and infection Vol. 9; no. 2; pp. 135 - 139
• Main Authors: Berg, H.F., Maraha, B., Bergmans, A.M.C., Zee, A. van der, Kluytmans, J.A.J.W., Peeters, M.F.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.02.2003; Blackwell Science Ltd; Blackwell
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Chlamydia Infections - pathology; Chlamydia pneumoniae; Chlamydophila pneumoniae; Chlamydophila pneumoniae - genetics; DNA extraction; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; Muscle, Smooth, Vascular - microbiology; Nucleic Acid Hybridization; Polymerase Chain Reaction - economics; Polymerase Chain Reaction - methods; Chlamydia pneumoniae; DNA extraction; Chlamydiaceae; Use; Method; Polymerase chain reaction; Tissue; DNA; Blood vessel; Chlamydiales; Bacteria; Extraction; Molecular biology; Comparative study
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1046/j.1469-0691.2003.00470.x

The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer—saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion—forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.