The bisphosphonate pamidronate on the surface of titanium stimulates bone formation around tibial implants in rats

• Published in: Biomaterials Vol. 26; no. 6; pp. 581 - 587
• Main Authors: Kajiwara, Hiroshi, Yamaza, Takayoshi, Yoshinari, Masao, Goto, Tetsuya, Iyama, Shinji, Atsuta, Ikiru, Kido, Mizuho A, Tanaka, Teruo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.02.2005
• Subjects: Animals; Bisphosphonate; Bone formation; Bone Resorption - prevention & control; Calcium; Coated Materials, Biocompatible - pharmacology; Diphosphonates - pharmacology; Diphosphonates - therapeutic use; Drug Evaluation, Preclinical; Implant; Implants, Experimental; Male; Materials Testing; Microscopy, Confocal; Osseointegration - drug effects; Rats; Rats, Wistar; Tibia - drug effects; Tibia - surgery; Tibia - ultrastructure; Titanium; Titanium; Bisphosphonate; Bone formation; Implant
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2004.02.072

Many materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. We analyzed the quantity of new bone formed in vivo around calcium-immobilized titanium implants with surfaces modified using pamidronate (PAM), a nitrogen-containing bisphosphonate (N-BP), implants of pure titanium, and titanium implants immobilized with calcium ions. New bone formation was visualized using fluorescent labeling (calcein blue and alizarin complexone) with intravenous injection at 1 and 3 weeks after implantation. After 4 weeks, undecalcified sections were prepared, and new bone formation around the implants was examined by morphometry using confocal laser scanning microscopy images. After 1 week, more new bone formed around the PAM-immobilized implant than around the calcium-immobilized and pure titanium implants. This was also seen with the new bone formation after 3 weeks. After 4 weeks, significantly more new bones were formed around the BP-immobilized implant than around the calcium ion-implanted and pure titanium implants. The new N-BP-modified titanium surface stimulates new bone formation around the implant, which might contribute to the success of implant therapy.