Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid

• Published in: Supportive care in cancer Vol. 22; no. 10; pp. 2741 - 2748
• Main Authors: Basso, Fernanda Gonçalves, Turrioni, Ana Paula Silveira, Soares, Diana Gabiela, Bagnato, Vanderlei Salvador, Hebling, Josimeri, de Souza Costa, Carlos Alberto
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2014; Springer; Springer Nature B.V
• Subjects: Bisphosphonate-Associated Osteonecrosis of the Jaw - therapy; Bone Density Conservation Agents - pharmacology; Bones; Cell culture; Collagen; Diphosphonates - pharmacology; Drug therapy; Gene expression; Humans; Imidazoles - pharmacology; Lasers; Lasers in medicine; Lasers in surgery; Low-Level Light Therapy - methods; Medicine; Medicine & Public Health; Nursing; Nursing Research; Oncology; Original Article; Osteoblasts - drug effects; Osteoblasts - physiology; Pain Medicine; Rehabilitation Medicine; Tumor Cells, Cultured; Wound healing; Low-level laser therapy; Cell culture; Osteonecrosis; Bisphosphonates; Osteoblasts
• ISSN: 0941-4355; 1433-7339
• DOI: 10.1007/s00520-014-2267-3

Purpose Clinical studies have shown that low-level laser therapy (LLLT) can improve local tissue healing of bisphosphonate-induced osteonecrosis of the jaw. However, the effects of laser irradiation on bisphosphonate-treated osteoblasts have not been completely elucidated. Methods Human osteoblasts were cultured in plain culture medium (DMEM). After 48 h, plain DMEM was replaced by DMEM with no fetal bovine serum, for a 24-h incubation followed by addition of zoledronic acid (5 μM) for additional 48 h. Cells were subjected to LLLT (InGaAsP; 780 ± 3 nm; 0.025 W) at 0.5, 1.5, 3, 5, and 7 J/cm 2 , three times every 24 h. Cell viability, total protein production, alkaline phosphatase activity (ALP), mineral nodule formation, gene expression of collagen type I and ALP, and cell morphology were evaluated. Results LLLT at 0.5 J/cm 2 increased cell viability of cultured osteoblasts. ALP activity and gene expression, in addition to mineral nodule formation and Col-I gene expression, were not increased by LLLT. LLLT applied to ZA-treated cells increased Col-I expression at 0.5, 1.5, and 3 J/cm 2 but did not improve any other cell activity assessed. Conclusion LLLT showed limited effects on bisphosphonate-treated osteoblasts.