Isolation and extraction of antimicrobial substances against oral bacteria from lemon peel

• Published in: Journal of food science and technology Vol. 48; no. 5; pp. 635 - 639
• Main Authors: Miyake, Yoshiaki, Hiramitsu, Masanori
• Format: Journal Article
• Language: English
• Published: India Springer-Verlag 01.10.2011; Springer Nature B.V
• Subjects: Bacteria; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Citrus fruits; Dental caries; Food; Food Science; Gum disease; Nutrition; Oils & fats; Short Communication; Solvents; Statistical analysis; 5-geranyloxy-7-methoxycoumarin; Lemon peel; 8-geranyloxypsolaren; 5-geranyloxypsolaren; Oral bacteria; Phlorin; Antibacterial substance
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-011-0330-3

We have isolated 4 antibacterial substances that were active against the oral bacteria that cause dental caries and periodontitis, such as Streptococcus mutans , Prevotella intermedia , and Porphyromonas gingivalis , from lemon peel, a waste product in the citrus industry. The isolated substances were identified as 8-geranyloxypsolaren, 5-geranyloxypsolaren, 5-geranyloxy-7-methoxycoumarin, and phloroglucinol 1-β-D-glucopyranoside (phlorin) upon structural analyses. Among these, 8-Geranyloxypsolaren, 5-geranyloxypsolaren, and 5-geranyloxy-7-methoxycoumarin exhibited high antibacterial activity. These 3 compounds were effectively extracted using ethanol and n-hexane, whereas phlorin was extracted with water. Further, the above 3 compounds were present in lemon essential oil and abundantly present in the residue produced upon the cooling treatment of concentrated lemon essential oil.