Intrahepatic growth and maturation of Gnathostoma turgidum in the natural definitive opossum host, Didelphis virginiana

• Published in: Parasitology international Vol. 59; no. 3; pp. 338 - 343
• Main Authors: Díaz-Camacho, Sylvia Páz, Delgado-Vargas, Francisco, Willms, Kaethe, del Carmen de la Cruz-Otero, María, Guadalupe Rendón-Maldonado, José, Robert, Lilia, Antuna, Silvia, Nawa, Yukifumi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.09.2010
• Subjects: Advanced 3rd stage larvae; Animals; Didelphis - parasitology; Didelphis virginiana; DNA, Helminth - analysis; DNA, Helminth - isolation & purification; DNA, Ribosomal Spacer - analysis; Gastroenterology and Hepatology; Gnathostoma - classification; Gnathostoma - genetics; Gnathostoma - growth & development; Gnathostoma - ultrastructure; Gnathostoma turgidum; Host-Parasite Interactions; Infectious Disease; Larva - growth & development; Larva - ultrastructure; Liver - parasitology; Microscopy, Electron, Scanning; Opossum; Polymerase Chain Reaction - methods; Sequence Analysis, DNA; Spirurida Infections - parasitology; Spirurida Infections - veterinary; Stomach - parasitology; Advanced 3rd stage larvae; Didelphis virginiana; Opossum; Gnathostoma turgidum
• ISSN: 1383-5769; 1873-0329
• DOI: 10.1016/j.parint.2010.04.004

Abstract Gnathostoma turgidum is a gastric nematode parasite of opossums found in the Americas. We recently found that G. turgidum juveniles appear in the liver of the opossums where they become mature adults and almost synchronously move to the stomach during certain months of the year, suggesting the importance of the liver for the growth and maturation of this species in the final hosts. In this study we attempted to detect G. turgidum larvae in the liver of opossums, Didelphis virginiana that are the natural final hosts. The results show that tiny (< 3 mm in length) third stage larvae (L3) appeared in the liver of opossums around November and December. Also in the liver, we found large L3 of up to about 10 mm in length together with juveniles and mature adults from February to March. In spite of their length, large L3 have 4 rows of hooklets, and their gonads remained undeveloped. Morphological features of the small and large L3 of G. turgidum are described including scanning electron microscope images. The seasonal switching of the several growth stages of G. turgidum from small L3 to adult worms in the liver and eventual migration to the stomach in opossums suggests the unique feature of G. turgidum utilizing the liver as the maturation site.